Structure of PDB 3hcf Chain A

Receptor sequence
>3hcfA (length=257) Species: 9606 (Homo sapiens) [Search protein sequence]
ASAYQRFEPRAYLRNNYAPPRGDLCNPNGVGPWKLRCLAQTFATGEVSGR
TLIDIGSGPTVYQLLSACSHFEDITMTDFLEVNRQELGRWLQEEPGAFNW
SMYSQHACLIEGKGECWQDKERQLRARVKRVLPIDVHQPQPLGAGSPAPL
PADALVSAFCLEAVSPDLASFQRALDHITTLLRPGGHLLLIGALEESWYL
AGEARLTVVPVSEEEVREALVRSGYKVRDLRTYIMPAHLQTGVDDVKGVF
FAWAQKV
3D structure
PDB3hcf Molecular recognition of physiological substrate noradrenaline by the adrenaline-synthesizing enzyme PNMT and factors influencing its methyltransferase activity.
ChainA
Resolution2.702 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.28: phenylethanolamine N-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 LT5 A R44 F182 E219 Y222 M258 R21 F159 E196 Y199 M235 BindingDB: Kd=2400nM
BS02 SAH A Y27 Y35 Y40 S80 T83 Y85 D101 F102 D158 V159 H160 A181 F182 C183 V187 Y4 Y12 Y17 S57 T60 Y62 D78 F79 D135 V136 H137 A158 F159 C160 V164
Gene Ontology
Molecular Function
GO:0004603 phenylethanolamine N-methyltransferase activity
GO:0005515 protein binding
GO:0008168 methyltransferase activity
Biological Process
GO:0032259 methylation
GO:0042418 epinephrine biosynthetic process
GO:0042423 catecholamine biosynthetic process
Cellular Component
GO:0005829 cytosol

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Biological Process
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Cellular Component
External links
PDB RCSB:3hcf, PDBe:3hcf, PDBj:3hcf
PDBsum3hcf
PubMed19570037
UniProtP11086|PNMT_HUMAN Phenylethanolamine N-methyltransferase (Gene Name=PNMT)

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