Structure of PDB 3guh Chain A

Receptor sequence

>3guhA (length=477) Species: 83333 (Escherichia coli K-12)

MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAFPDIRRG
VTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNL
FAYTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAAR
GRPAKSVFTVHNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKA
GLYYADHITAVSPTYAREITEPQFAYGMEGLLQQRHREGRLSGVLNGVDE
KIWSPETDLLLASRYTRDTLEDKAENKRQLQIAMGLKVDDKVPLFAVVSR
LTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVLQEGFLAAAAEYPGQVG
VQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTLPLVRRTGG
LADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
VQRQAMAMDFSWQVAAKSYRELYYRLK

3D structure

• PDB: 3guh The Crystal Structures of the Open and Catalytically Competent Closed Conformation of Escherichia coli Glycogen Synthase.
• Chain: A
• Resolution: 2.79 Å

Enzymatic activity

• Enzyme Commision number: 2.4.1.21: starch synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	A	K15 G18 S299 R300 K305 L327 Y355 H356 G380 L381 T382 Y385	K15 G18 S299 R300 K305 L327 Y355 H356 G380 L381 T382 Y385
BS02	250	A	L19 Y95 W138 H139 Y165	L19 Y95 W138 H139 Y165
BS03	ASO	A	G18 H161 V211 N246 E377 P378 C379 G380	G18 H161 V211 N246 E377 P378 C379 G380

Gene Ontology

Molecular Function

• GO:0004373 alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity
• GO:0009011 alpha-1,4-glucan glucosyltransferase (ADP-glucose donor) activity
• GO:0016757 glycosyltransferase activity

Biological Process

• GO:0005978 glycogen biosynthetic process
• GO:0006974 DNA damage response

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:3guh, PDBe:3guh, PDBj:3guh
• PDBsum: 3guh
• PubMed: 19244233
• UniProt: P0A6U8|GLGA_ECOLI Glycogen synthase (Gene Name=glgA)