Structure of PDB 3gsi Chain A

Receptor sequence

>3gsiA (length=827) Species: 1665 (Arthrobacter globiformis) [Search protein sequence]

TPRIVIIGAGIVGTNLADELVTRGWNNITVLDQGPLNMPGGSTSHAPGLV
FQTNPSKTMASFAKYTVEKLLSLTEDGVSCFNQVGGLEVATTETRLADLK
RKLGYAAAWGIEGRLLSPAECQELYPLLDGENILGGLHVPSDGLASAARA
VQLLIKRTESAGVTYRGSTTVTGIEQSGGRVTGVQTADGVIPADIVVSCA
GFWGAKIGAMIGMAVPLLPLAHQYVKTTPVPAQQGRNDQPNGARLPILRH
QDQDLYYREHGDRYGIGSYAHRPMPVDVDTLGAYAPETVSEHHMPSRLDF
TLEDFLPAWEATKQLLPALADSEIEDGFNGIFSFTPDGGPLLGESKELDG
FYVAEAVWVTHSAGVAKAMAELLTTGRSETDLGECDITRFEDVQLTPEYV
SETSQQNFVEIYDVLHPLQPRLSPRNLRVSPFHARHKELGAFFLEAGGWE
RPYWFEANAALLKEMPAEWLPPARDAWSGMFSSPIAAAEAWKTRTAVAMY
DMTPLKRLEVSGPGALKLLQELTTADLAKKPGAVTYTLLLDHAGGVRSAI
TVARLSEDTFQLGANGNIDTAYFERAARHQTQSGSATDWVQVRDTTGGTC
CIGLWGPLARDLVSKVSDDDFTNDGLKYFRAKNVVIGGIPVTAMRLSYVG
ELGWELYTSADNGQRLWDALWQAGQPFGVIAAGRAAFSSLRLEKGYRSWG
TDMTTEHDPFEAGLGFAVKMAKESFIGKGALEGRTEEASARRLRCLTIDD
GRSIVLGKEPVFYKEQAVGYVTSAAYGYTVAKPIAYSYLPGTVSVGDSVD
IEYFGRRITATVTEDPLYDPKMTRLRG

3D structure

PDB

3gsi An internal reaction chamber in dimethylglycine oxidase provides efficient protection from exposure to toxic formaldehyde.

Chain

Resolution

2.0 Å

3D
structure

Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]

Enzymatic activity

Catalytic site (original residue number in PDB)	H225 Y259 A552
Catalytic site (residue number reindexed from 1)	H222 Y256 A549
Enzyme Commision number	1.5.3.10: dimethylglycine oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	THG	A	L508 Y539 T554 G566 F632 Y651 E658 Y699	L505 Y536 T551 G563 F629 Y648 E655 Y696
BS02	MG	A	D257 V412	D254 V409
BS03	FAD	A	G13 I14 V15 D35 Q36 G44 S45 T46 H48 P50 L52 T173 V174 A203 G204 W206 H225 Y259 G333 I334 F335 V360 W361 V362 T363	G10 I11 V12 D32 Q33 G41 S42 T43 H45 P47 L49 T170 V171 A200 G201 W203 H222 Y256 G330 I331 F332 V357 W358 V359 T360

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0016491	oxidoreductase activity
GO:0047866	dimethylglycine oxidase activity

View graph for
Molecular Function

External links

PDB	RCSB:3gsi, PDBe:3gsi, PDBj:3gsi
PDBsum	3gsi
PubMed	19369258
UniProt	Q9AGP8\|DMGO_ARTGO Dimethylglycine oxidase (Gene Name=dmg)

[Back to BioLiP]