Structure of PDB 3gf4 Chain A

Receptor sequence

>3gf4A (length=383) Species: 573 (Klebsiella pneumoniae)

KSKKILIVGAGFSGAVIGRQLAEKGHQVHIIDQRDHIGGNSYDARDSETN
VMVHVYGPHIFHTDNETVWNYINKHAEMMPYVNRVKATVNGQVFSLPINL
HTINQFFSKTCSPDEARALIAEKGDSTIADPQTFEEQALRFIGKELYEAF
FKGYTIKQWGMQPSELPASILKRLPVRFNYDDNYFNHKFQGMPKCGYTQM
IKSILNHENIKVDLQREFIVDERTHYDHVFYSGPLDAFYGYQYGRLGYRT
LDFKKFIYQGDYQGCAVMNYCSVDVPYTRITEHKYFSPWEQHDGSVCYKE
YSRACEENDIPYYPIRQMGEMALLEKYLSLAENETNITFVGRLGTYRYLD
MDVTIAEALKTAEVYLNSLTDNQPMPVFTVSVG

3D structure

• PDB: 3gf4 Ligand binding and substrate discrimination by UDP-galactopyranose mutase.
• Chain: A
• Resolution: 2.45 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R174 R178 R250 R280 E301 Y349 D351
• Catalytic site (residue number reindexed from 1): R173 R177 R249 R279 E300 Y348 D350
• Enzyme Commision number: 5.4.99.9: UDP-galactopyranose mutase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	V9 G10 G12 S14 D33 Q34 R35 G40 H60 I61 F219 G234 Y313 R343 Y349 L350 D351 M352 T355	V8 G9 G11 S13 D32 Q33 R34 G39 H59 I60 F218 G233 Y312 R342 Y348 L349 D350 M351 T354
BS02	UPG	A	F152 Y155 T156 Q159 W160 L175 Y185 N270 R280 Y314	F151 Y154 T155 Q158 W159 L174 Y184 N269 R279 Y313	MOAD: Kd=750uM

Gene Ontology

Molecular Function

• GO:0008767 UDP-galactopyranose mutase activity

External links

• PDB: RCSB:3gf4, PDBe:3gf4, PDBj:3gf4
• PDBsum: 3gf4
• PubMed: 19500588
• UniProt: Q48485|GLF1_KLEPN UDP-galactopyranose mutase (Gene Name=rfbD)