Structure of PDB 3g5s Chain A

Receptor sequence

>3g5sA (length=424) Species: 300852 (Thermus thermophilus HB8)

MERVNVVGAGLAGSEAAWTLLRLGVPVRLFEMRPKRMTPAHGTDRFAEIV
CSNSLGGEGETNAKGLLQAEMRRAGSLVMEAADLARVPAGGALAVDREEF
SGYITERLTGHPLLEVVREEVREIPPGITVLATGPLTSEALAEALKRRFG
DHFLAYYDAASPIVLYESIDLTKCFRAGRYYLNCPMTEEEYRRFHQALLE
AQRHTPHFEACVPVEELARRGYQTLLFGPMKPVGLVDPRTGKEPFAVVQL
RQEDKAGRMWSLVGFQTGLKWPEQKRLIQMIPGLENAEIVRYGVMHRNTY
LNAPRLLGETLEFREAEGLYAAGVLAGVEGYLESAATGFLAGLNAARKAL
GLPPVAPPEESMLGGLVRYLATANPEGFQPMYANWGLVPPVEGRMGKKEK
RQAMYRRGLEAFSAWLSGLNPPLP

3D structure

• PDB: 3g5s Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase
• Chain: A
• Resolution: 1.05 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.74: methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-methyltransferase [NAD(P)H-oxidizing].

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	G8 G10 L11 A12 E31 M32 R33 C51 S52 E120 V121 T133 G134 P135 S138 H212 G335 V336 E341 G342 Y343 S346	G8 G10 L11 A12 E31 M32 R33 C51 S52 E120 V121 T133 G134 P135 S138 H207 G323 V324 E329 G330 Y331 S334
BS02	GSH	A	A155 A159 S161 C223 Q278 Y312 G389 Q391	A155 A159 S161 C211 Q266 Y300 G377 Q379

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity
• GO:0047151 tRNA (uracil(54)-C5)-methyltransferase activity, 5,10-methylenetetrahydrofolate-dependent
• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0002098 tRNA wobble uridine modification
• GO:0008033 tRNA processing
• GO:0030488 tRNA methylation
• GO:0032259 methylation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:3g5s, PDBe:3g5s, PDBj:3g5s
• PDBsum: 3g5s
• PubMed: 19416846
• UniProt: Q5SID2|TRMFO_THET8 Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO (Gene Name=trmFO)