Structure of PDB 3fz8 Chain A

Receptor sequence

>3fz8A (length=455) Species: 83333 (Escherichia coli K-12)

ELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQIINDELYLDGNARQNLA
TFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAP
APKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGP
VQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGV
TYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWD
FRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIG
TFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLG
PYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTL
GGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQN
SFKHT

3D structure

• PDB: 3fz8 Crystal structure of glutamate decarboxylase beta from Escherichia coli: reduced Schiff base with PLP
• Chain: A
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 4.1.1.15: glutamate decarboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PLR	A	S126 S127 Q163 T212 D243 A245 S273 H275 K276 T466	S115 S116 Q152 T201 D232 A234 S262 H264 K265 T455

Gene Ontology

Molecular Function

• GO:0004351 glutamate decarboxylase activity
• GO:0005515 protein binding
• GO:0016830 carbon-carbon lyase activity
• GO:0016831 carboxy-lyase activity
• GO:0030170 pyridoxal phosphate binding

Biological Process

• GO:0006536 glutamate metabolic process
• GO:0006538 glutamate catabolic process
• GO:0019752 carboxylic acid metabolic process
• GO:0051454 intracellular pH elevation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0016020 membrane

External links

• PDB: RCSB:3fz8, PDBe:3fz8, PDBj:3fz8
• PDBsum: 3fz8
• UniProt: P69910|DCEB_ECOLI Glutamate decarboxylase beta (Gene Name=gadB)