Structure of PDB 3fux Chain A

Receptor sequence
>3fuxA (length=264) Species: 300852 (Thermus thermophilus HB8) [Search protein sequence]
ASPQSVRALLERHGLFADKRFGQNFLVSEAHLRRIVEAARPFTGPVFEVG
PGLGALTRALLEAGAEVTAIEKDLRLRPVLEETLSGLPVRLVFQDALLYP
WEEVPQGSLLVANLPYHIATPLVTRLLKTGRFARLVFLVQKEVAERMTAR
PKTPAYGVLTLRVAHHAVAERLFDLPPGAFFPPPKVWSSLVRLTPTGALD
DPGLFRLVEAAFGKRRKTLLNALAAAGYPKARVEEALRALGLPPRVRAEE
LDLEAFRRLREGLE
3D structure
PDB3fux Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5'-methylthioadenosine.
ChainA
Resolution1.68 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) G54 E75 N117 Y120 F184
Catalytic site (residue number reindexed from 1) G50 E71 N113 Y116 F180
Enzyme Commision number 2.1.1.182: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MTA A F25 G26 Q27 N28 G54 G56 E75 K76 D99 A100 N117 P119 F21 G22 Q23 N24 G50 G52 E71 K72 D95 A96 N113 P115
Gene Ontology
Molecular Function
GO:0000179 rRNA (adenine-N6,N6-)-dimethyltransferase activity
GO:0003723 RNA binding
GO:0008168 methyltransferase activity
GO:0016433 rRNA (adenine) methyltransferase activity
GO:0052908 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity
Biological Process
GO:0000154 rRNA modification
GO:0006364 rRNA processing
GO:0031167 rRNA methylation
GO:0032259 methylation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:3fux, PDBe:3fux, PDBj:3fux
PDBsum3fux
PubMed19285505
UniProtQ5SM60|RSMA_THET8 Ribosomal RNA small subunit methyltransferase A (Gene Name=rsmA)

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