Structure of PDB 3fmt Chain A

Receptor sequence

>3fmtA (length=162) Species: 83333 (Escherichia coli K-12)

MKTIEVDDELYSYIASHTKHIGESRSDILRRMLKFSAASQKPVKTIKDKV
RAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLDAQAFAEATESLHGRTRV
YFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMIEHIMQSMQF
PAELIEKVCGTI

3D structure

• PDB: 3fmt Structural insights into the cooperative binding of SeqA to a tandem GATC repeat
• Chain: A
• Resolution: 2.983 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R86 A87 V88 R116 K136 K138 N152 T153	R67 A68 V69 R97 K117 K119 N133 T134
BS02	dna	A	G115 R116 T117 R118 Y120 Q134 N150 R155	G96 R97 T98 R99 Y101 Q115 N131 R136

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003688 DNA replication origin binding
• GO:0005515 protein binding
• GO:0010385 double-stranded methylated DNA binding
• GO:0042802 identical protein binding
• GO:0042803 protein homodimerization activity
• GO:0043565 sequence-specific DNA binding
• GO:0044729 hemi-methylated DNA-binding

Biological Process

• GO:0006355 regulation of DNA-templated transcription
• GO:0007062 sister chromatid cohesion
• GO:0008156 negative regulation of DNA replication
• GO:0009314 response to radiation
• GO:0032297 negative regulation of DNA-templated DNA replication initiation
• GO:0090143 nucleoid organization

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0032991 protein-containing complex
• GO:1990097 SeqA-DNA complex

External links

• PDB: RCSB:3fmt, PDBe:3fmt, PDBj:3fmt
• PDBsum: 3fmt
• PubMed: 19304745
• UniProt: P0AFY8|SEQA_ECOLI Negative modulator of initiation of replication (Gene Name=seqA)