Structure of PDB 3fcf Chain A

Receptor sequence
>3fcfA (length=223) Species: 9606 (Homo sapiens) [Search protein sequence]
MEFFGESWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQM
CDIKDVKVVILGQDPYHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDI
EDFVHPGHGDLSGWAKQGVLLLNAVLTVRAHQANSHKERGWEQFTDAVVS
WLNQNSNGLVFLLWGSYAQKKGSAIDRKRHHVLQTAHPSPLSVYRGFFGC
RHFSKTNELLQKSGKKPIDWKEL
3D structure
PDB3fcf Impact of linker strain and flexibility in the design of a fragment-based inhibitor
ChainA
Resolution1.84 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D145 Y147 F158 H268
Catalytic site (residue number reindexed from 1) D64 Y66 F77 H187
Enzyme Commision number 3.2.2.27: uracil-DNA glycosylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FCF A G143 Q144 D145 Y147 C157 F158 S169 N204 G246 S247 H268 S270 S273 G62 Q63 D64 Y66 C76 F77 S88 N123 G165 S166 H187 S189 S192 MOAD: ic50=40uM
PDBbind-CN: -logKd/Ki=4.40,IC50=40uM
BindingDB: IC50=4.0e+4nM
Gene Ontology
Molecular Function
GO:0004844 uracil DNA N-glycosylase activity
GO:0016799 hydrolase activity, hydrolyzing N-glycosyl compounds
Biological Process
GO:0006281 DNA repair
GO:0006284 base-excision repair

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Molecular Function
View graph for
Biological Process
External links
PDB RCSB:3fcf, PDBe:3fcf, PDBj:3fcf
PDBsum3fcf
PubMed19396178
UniProtP13051|UNG_HUMAN Uracil-DNA glycosylase (Gene Name=UNG)

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