Structure of PDB 3f9m Chain A

Receptor sequence
>3f9mA (length=451) Species: 9606 (Homo sapiens) [Search protein sequence]
ENLYFQGMKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEA
SVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEQWSVKTKHQM
YSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHE
DIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTV
ATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEW
GAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLL
RLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGL
RPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVG
VDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVAC
K
3D structure
PDB3f9m The active conformation of human glucokinase is not altered by allosteric activators
ChainA
Resolution1.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R85 S151 N166 K169 D205
Catalytic site (residue number reindexed from 1) R82 S144 N159 K162 D198
Enzyme Commision number 2.7.1.1: hexokinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GLC A S151 F152 T168 K169 N204 D205 I225 N231 E256 E290 S144 F145 T161 K162 N197 D198 I218 N224 E249 E283
BS02 MRK A Y61 V62 R63 S64 T65 I211 Y214 Y215 M235 V452 V455 Y58 V59 R60 S61 T62 I204 Y207 Y208 M228 V445 V448 BindingDB: EC50=67.11nM,Kd=360nM
Gene Ontology
Molecular Function
GO:0004340 glucokinase activity
GO:0004396 hexokinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0005536 D-glucose binding
GO:0008865 fructokinase activity
GO:0016301 kinase activity
GO:0016773 phosphotransferase activity, alcohol group as acceptor
GO:0019158 mannokinase activity
GO:0141089 glucose sensor activity
Biological Process
GO:0001678 intracellular glucose homeostasis
GO:0005975 carbohydrate metabolic process
GO:0006006 glucose metabolic process
GO:0006007 glucose catabolic process
GO:0006096 glycolytic process
GO:0006110 regulation of glycolytic process
GO:0006739 NADP metabolic process
GO:0009749 response to glucose
GO:0016310 phosphorylation
GO:0019318 hexose metabolic process
GO:0032024 positive regulation of insulin secretion
GO:0032869 cellular response to insulin stimulus
GO:0042593 glucose homeostasis
GO:0043266 regulation of potassium ion transport
GO:0044320 cellular response to leptin stimulus
GO:0045721 negative regulation of gluconeogenesis
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0046835 carbohydrate phosphorylation
GO:0050796 regulation of insulin secretion
GO:0051156 glucose 6-phosphate metabolic process
GO:0061621 canonical glycolysis
GO:0070509 calcium ion import
GO:1901135 carbohydrate derivative metabolic process
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3f9m, PDBe:3f9m, PDBj:3f9m
PDBsum3f9m
PubMed22101819
UniProtP35557|HXK4_HUMAN Hexokinase-4 (Gene Name=GCK)

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