Structure of PDB 3f5f Chain A

Receptor sequence

>3f5fA (length=656) Species: 9031,83333 [Search protein sequence]

KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQV
AATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYN
GKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFN
LQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIK
NKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLG
AVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINA
ASGRQTVDAALAAAQTNAAADEEDDVVIIYNRVPKTASTSFTNIAYDLCA
KNRYHVLHINTTKNNPVMSLQDQVRFVKNVTSWKEMKPGFYHGHVSYLDF
AKFGVKKKPIYINVIRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTF
DECVAAGGSDCAPEKLWLQIPFFCGHSSECWNVGSRWALEQAKYNLINEY
FLVGVTEELEDFIMLLEAALPRFFRGATELYRTGKKSHLRKTTEKKLPTA
ATIAKLQQSEIWKMENEFYEFALEQFQFVRAHAVREKDGELYILAQNFFY
EKIYPK

3D structure

PDB

3f5f Redirecting the substrate specificity of heparan sulfate 2-O-sulfotransferase by structurally guided mutagenesis.

Chain

Resolution

2.65 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.8.2.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	GLC	A	D14 K15 E111 Y155 W230	D14 K15 E111 Y155 W230
BS02	GLC	A	W62 D65 R66 E153 P154 Y155 W340 R344	W62 D65 R66 E153 P154 Y155 W340 R344
BS03	A3P	A	K1083 T1084 A1085 S1086 T1087 S1088 R1164 S1172 Y1279 S1285 H1286 R1288 K1289 T1290 K1293	K385 T386 A387 S388 T389 S390 R466 S474 Y581 S587 H588 R590 K591 T592 K595

Gene Ontology

	Molecular Function
GO:0005515	protein binding
GO:0008146	sulfotransferase activity
GO:0015144	carbohydrate transmembrane transporter activity
GO:1901982	maltose binding

	Biological Process
GO:0006974	DNA damage response
GO:0008643	carbohydrate transport
GO:0015768	maltose transport
GO:0034219	carbohydrate transmembrane transport
GO:0034289	detection of maltose stimulus
GO:0042956	maltodextrin transmembrane transport
GO:0055085	transmembrane transport
GO:0060326	cell chemotaxis

	Cellular Component
GO:0016020	membrane
GO:0030288	outer membrane-bounded periplasmic space
GO:0042597	periplasmic space
GO:0043190	ATP-binding cassette (ABC) transporter complex
GO:0055052	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
GO:1990060	maltose transport complex

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Molecular Function

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Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:3f5f, PDBe:3f5f, PDBj:3f5f
PDBsum	3f5f
PubMed	19022906
UniProt	P0AEX9\|MALE_ECOLI Maltose/maltodextrin-binding periplasmic protein (Gene Name=malE); Q76KB1

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