Structure of PDB 3ezw Chain A

Receptor sequence
>3ezwA (length=498) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
KKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDPMEI
WATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIV
WQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSR
ERARRGELLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDK
MLEVLDIPREMLPEVRRSSEVYGQTNIDGKGGTRIPISGIAGDQQAALFG
QLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGEVNYAL
EGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLG
APYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIR
LHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVG
FWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHD
3D structure
PDB3ezw Crystal structure of a hyperactive Escherichia coli glycerol kinase mutant Gly230 --> Asp obtained using microfluidic crystallization devices.
ChainA
Resolution2.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.1.30: glycerol kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GOL A R83 E84 W103 Y135 D245 F270 R81 E82 W101 Y133 D243 F268
Gene Ontology
Molecular Function
GO:0004370 glycerol kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016301 kinase activity
GO:0016773 phosphotransferase activity, alcohol group as acceptor
GO:0042802 identical protein binding
GO:0046872 metal ion binding
Biological Process
GO:0005975 carbohydrate metabolic process
GO:0006071 glycerol metabolic process
GO:0006072 glycerol-3-phosphate metabolic process
GO:0006974 DNA damage response
GO:0016310 phosphorylation
GO:0019563 glycerol catabolic process
Cellular Component
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:3ezw, PDBe:3ezw, PDBj:3ezw
PDBsum3ezw
PubMed17441732
UniProtP0A6F3|GLPK_ECOLI Glycerol kinase (Gene Name=glpK)

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