Structure of PDB 3ex2 Chain A

Receptor sequence
>3ex2A (length=257) Species: 9606 (Homo sapiens) [Search protein sequence]
MELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADA
LGPSICMLKTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTV
KKQYEGGIFKIASWADLVNAHVVPGSGVVKGLQEVGLPLHRGCLLIAEMS
STGSLATGDYTRAAVRMAEEHSEFVVGFISGSRVSMKPEFLHLTPGVQLE
AGGDNLGQQYNSPQEVIGKRGSDIIIVGRGIISAADRLEAAEMYRKAAWE
AYLSRLG
3D structure
PDB3ex2 Lys314 is a nucleophile in non-classical reactions of orotidine-5'-monophosphate decarboxylase
ChainA
Resolution1.55 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.4.2.10: orotate phosphoribosyltransferase.
4.1.1.23: orotidine-5'-phosphate decarboxylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 6CN A S257 D259 K281 H283 D312 K314 M371 S372 P417 Q430 Y432 G450 R451 S35 D37 K59 H61 D90 K92 M149 S150 P195 Q208 Y210 G228 R229 MOAD: ic50~200uM
PDBbind-CN: -logKd/Ki=3.70,IC50=200uM
BS02 6CN A D317 I318 T321 D95 I96 T99 MOAD: ic50~200uM
PDBbind-CN: -logKd/Ki=3.70,IC50=200uM
Gene Ontology
Molecular Function
GO:0004590 orotidine-5'-phosphate decarboxylase activity
Biological Process
GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process
GO:0044205 'de novo' UMP biosynthetic process

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Molecular Function
View graph for
Biological Process
External links
PDB RCSB:3ex2, PDBe:3ex2, PDBj:3ex2
PDBsum3ex2
PubMed19472232
UniProtP11172|UMPS_HUMAN Uridine 5'-monophosphate synthase (Gene Name=UMPS)

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