Structure of PDB 3ewu Chain A

Receptor sequence
>3ewuA (length=257) Species: 9606 (Homo sapiens) [Search protein sequence]
MELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADA
LGPSICMLKTHVDILNDFTLDVMKELITLAKCHEFLIFENRKFADIGNTV
KKQYEGGIFKIASWADLVNAHVVPGSGVVKGLQEVGLPLHRGCLLIAEMS
STGSLATGDYTRAAVRMAEEHSEFVVGFISGSRVSMKPEFLHLTPGVQLE
AGGDNLGQQYNSPQEVIGKRGSDIIIVGRGIISAADRLEAAEMYRKAAWE
AYLSRLG
3D structure
PDB3ewu Lys314 is a nucleophile in non-classical reactions of orotidine-5'-monophosphate decarboxylase
ChainA
Resolution1.6 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.4.2.10: orotate phosphoribosyltransferase.
4.1.1.23: orotidine-5'-phosphate decarboxylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 UEP A S257 D259 N312 K314 M371 S372 P417 Q430 Y432 G450 R451 S35 D37 N90 K92 M149 S150 P195 Q208 Y210 G228 R229 PDBbind-CN: -logKd/Ki=4.62,IC50=24uM
BS02 UEP A D317 I318 T321 D95 I96 T99 PDBbind-CN: -logKd/Ki=4.62,IC50=24uM
Gene Ontology
Molecular Function
GO:0004590 orotidine-5'-phosphate decarboxylase activity
Biological Process
GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process
GO:0044205 'de novo' UMP biosynthetic process

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Molecular Function
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Biological Process
External links
PDB RCSB:3ewu, PDBe:3ewu, PDBj:3ewu
PDBsum3ewu
PubMed19472232
UniProtP11172|UMPS_HUMAN Uridine 5'-monophosphate synthase (Gene Name=UMPS)

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