Structure of PDB 3etg Chain A

Receptor sequence

>3etgA (length=501) Species: 9913 (Bos taurus)

ADREDDPNFFKMVEGFFDRGASIVEDKLVEDLKTRETEEQKRNRVRGILR
IIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSQHRTPCKGGIRYSTDVS
VDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDNELEKITRRFTM
ELAKKGFIGPGVDVPAPDMSTGEREMSWIADTYASTIGHYDINAHACVTG
KPISQGGIHGRISATGRGVFHGIENFINEASYMSILGMTPGFGDKTFAVQ
GFGNVGLHSMRYLHRFGAKCVAVGESDGSIWNPDGIDPKELEDFKLQHGT
ILGFPKAKIYEGSILEVDCDILIPAASEKQLTKSNAPRVKAKIIAEGANG
PTTPEADKIFLERNIMVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFK
YERDSNYHLLMSVQESLERKFGKHGGTIPIVPTAEFQDRISGASEKDIVH
SGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFRVYNEAGVTF
T

3D structure

• PDB: 3etg Novel Inhibitors Complexed with Glutamate Dehydrogenase: ALLOSTERIC REGULATION BY CONTROL OF PROTEIN DYNAMICS
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K126 D168
• Catalytic site (residue number reindexed from 1): K126 D168
• Enzyme Commision number: 1.4.1.3: glutamate dehydrogenase [NAD(P)(+)].

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLU	A	K90 M111 K114 A166 V378 S381	K90 M111 K114 A166 V378 S381
BS02	NDP	A	D168 M169 R211 T215 Q250 G253 V255 E275 S276 A326 G347 A348 N349 N374	D168 M169 R211 T215 Q250 G253 V255 E275 S276 A326 G347 A348 N349 N374
BS03	GTP	A	H209 G210 S213 R217 R261 R265 E292	H209 G210 S213 R217 R261 R265 E292
BS04	GWD	A	R146 R147 M150	R146 R147 M150	MOAD: ic50=1.5uM

Gene Ontology

Molecular Function

• GO:0004352 glutamate dehydrogenase (NAD+) activity
• GO:0004353 glutamate dehydrogenase [NAD(P)+] activity
• GO:0004354 glutamate dehydrogenase (NADP+) activity
• GO:0005524 ATP binding
• GO:0005525 GTP binding
• GO:0016491 oxidoreductase activity
• GO:0016639 oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor
• GO:0042802 identical protein binding

Biological Process

• GO:0006520 amino acid metabolic process
• GO:0006538 glutamate catabolic process
• GO:0006541 glutamine metabolic process
• GO:0072350 tricarboxylic acid metabolic process

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0005783 endoplasmic reticulum

External links

• PDB: RCSB:3etg, PDBe:3etg, PDBj:3etg
• PDBsum: 3etg
• PubMed: 19531491
• UniProt: P00366|DHE3_BOVIN Glutamate dehydrogenase 1, mitochondrial (Gene Name=GLUD1)