Structure of PDB 3esw Chain A

Receptor sequence
>3eswA (length=333) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
SSGLVPRGSHMNNIDFDSIAKMLLIKYKDFILSKFKKAAPVENIRFQNLV
HTNQFAQGVLGQSQHLCTVYDNPSWHSIVLETLDLDLIYKNVDKEFAKDG
HAEGENIYTDYLVKELLRYFKQDFFKWCNKPDCNHCGQNTSENMTPLGSQ
GPNGEESKFNCGTVEIYKCNRCGNITRFPRYNDPIKLLETRKGRCGEWCN
LFTLILKSFGLDVRYVWNREDHVWCEYFSNFLNRWVHVDSCEQSFDQPYI
YSINWNKKMSYCIAFGKDGVVDVSKRYILQNELPRDQIKEEDLKFLCQFI
TKRLRYSLNDDEIYQLACRDEQEQIELIRGKTQ
3D structure
PDB3esw Structural and mutational studies on the importance of oligosaccharide binding for the activity of yeast PNGase.
ChainA
Resolution3.4 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.5.1.52: peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A C132 C165 C168 C136 C169 C172
Gene Ontology
Molecular Function
GO:0000224 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0046872 metal ion binding
Biological Process
GO:0006515 protein quality control for misfolded or incompletely synthesized proteins
GO:0036503 ERAD pathway
GO:0097466 ubiquitin-dependent glycoprotein ERAD pathway
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0120125 PNGase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3esw, PDBe:3esw, PDBj:3esw
PDBsum3esw
PubMed18854368
UniProtQ02890|PNG1_YEAST Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (Gene Name=PNG1)

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