Structure of PDB 3efr Chain A

Receptor sequence
>3efrA (length=233) Species: 63363 (Aquifex aeolicus) [Search protein sequence]
MFKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWLSQEG
GLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQ
EKKVSGVLRELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDR
KEVLLKVLKRISENLKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKIT
GKLVGLSEKGGALILTEEGIKEILSGEFSLRRS
3D structure
PDB3efr Structural and functional studies of the biotin protein ligase from Aquifex aeolicus reveal a critical role for a conserved residue in target specificity.
ChainA
Resolution2.55 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) G40 K103 R231
Catalytic site (residue number reindexed from 1) G40 K103 R231
Enzyme Commision number 6.3.4.15: biotin--[biotin carboxyl-carrier protein] ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 BTN A S13 T14 Q15 Q34 G37 R38 G40 L46 Y53 F54 G106 L108 G119 I120 G121 S13 T14 Q15 Q34 G37 R38 G40 L46 Y53 F54 G106 L108 G119 I120 G121 PDBbind-CN: -logKd/Ki=5.08,Kd=8.3uM
Gene Ontology
Molecular Function
GO:0004077 biotin--[biotin carboxyl-carrier protein] ligase activity
GO:0005524 ATP binding
GO:0016874 ligase activity
Biological Process
GO:0036211 protein modification process
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3efr, PDBe:3efr, PDBj:3efr
PDBsum3efr
PubMed19385043
UniProtO66837

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