Structure of PDB 3edj Chain A

Receptor sequence

>3edjA (length=597) Species: 197856 (Flavobacterium sp. 92)

PTAIEHMEPPFWWAGMQHKGLQLMVHGRDIGRMEAALDYPGVRLVSPTRV
PNANYLFVDLEIGPEAQPGSFDIVFKGDGRSERYRYRLLAREQGSAQRQG
FGPGDAIYQIMPDRFANGDPSNDNVAGMREQADRRHGGGRHGGDIRGTID
HLDYIAGLGFTQLWPTPLVENDAAAYSYHGYAATDHYRIDPRYGSNEDFV
RLSTEARKRGMGLIQDVVLSHIGKHHWWMKDLPTPDWINYGGKFVPTQHH
RVAVQDPYAAQADSENFTKGWFVEGMPDLNQTNPLVANYLIQNNIWWIEY
AGLSGLRIDTYGYSDGAFLTEYTRRLMAEYPRLNMVGQEWSTRVPVVARW
QRGKANFDGYTSHLPSLMDFPLVDAMRNALSKTGEENGLNEVYETLSLDY
LYPEPQNLVLFGGNHDMARMFSAAGEDFDRWRMNLVFLMTMPRIPQFYSG
DEILMTSTVKGRDDASYRRDFPGGWAGDKANAFSGAGLTSQQRAAQDLVR
KLANWRKNQPVIHNGRLMHFGPEENTWVYFRYNKDKRIMVAMNNNDKPMT
LPTARFQEMLKGAPSGVDFLSGKTVGLGRELRLAPKSVVVIELPGLP

3D structure

• PDB: 3edj Structural base for enzymatic cyclodextrin hydrolysis
• Chain: A
• Resolution: 1.69 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D218 R309 D311 Q340 H417 D418
• Catalytic site (residue number reindexed from 1): D216 R307 D309 Q338 H415 D416
• Enzyme Commision number: 3.2.1.54: cyclomaltodextrinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	Y183 H223 D311 Q340 H417 D418	Y181 H221 D309 Q338 H415 D416
BS02	GLC	A	H181 M278 D466 R470	H179 M276 D464 R468
BS03	GLC	A	V275 R464 D466	V273 R462 D464
BS04	GLC	A	R253 F274 T312 Y315 Q340 R464	R251 F272 T310 Y313 Q338 R462
BS05	CA	A	S222 T270 D280 Y315	S220 T268 D278 Y313
BS06	CA	A	N119 D121 N124 D125 G144 D146	N117 D119 N122 D123 G142 D144

Gene Ontology

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:3edj, PDBe:3edj, PDBj:3edj
• PDBsum: 3edj
• PubMed: 19014948
• UniProt: Q8KKG0