Structure of PDB 3e0d Chain A

Receptor sequence

>3e0dA (length=1148) Species: 271 (Thermus aquaticus)

LKFAHLHQHTQFSLLNGAAKLQDLLKWVKETTPEDPALAMTDHGNLFGAV
EFYKKATAMGVKPIIGYEAYVAAESRFDRKRGGYFHLTLLAKDFTGYQNL
VRLASRAYLEGFYEKPRIDREILREHAQGLIALSGCLGAEIPQFILQDRL
DLAEARLNEDLSIFGDRFFIEIQNHGLPEQKKVNQVLKEFARKYGLGMVA
TNNGHYVRKEDARAHEVLLAIQSKTTLDDPERWRFPCDEFYVKTPEEMRA
MLPEAEWGDEPFDNTVEIARMCDVDLPIGDKMVYRIPRFPLGRTEAQYLR
ELTFLGLLRRYPDRITEAFYREVLRLLDERALAEALARVEEKAWEELRKR
EWTAEAILHRALYELSVIERMGFPGYFLIVQDYINWARGHGVSVGPGRGS
AAGSLVAYAVGITNIDPLRFGLLFERFLNPERVSMPDIDTDFSDRERDRV
IQYVRERYGEDKVAQIGTFGSLASKAALKDVARVYGIPHKKAEELAKLIP
VQFGKPKPLQEAFEAEPELRAEMEKDERIRQVIEVAMRLEGLNRHASVHA
AGVVIAAEPLTDLVPLMRDQEGRPVTQYDMGAVEALGLLKMDFLGLRTLT
FLDEARRIVKESKGVELDYDRLPLDDPKTFELLSRGETKGVFQLESGGMT
ATVRGLKPRRLEDIIALVSLYRPGPMEHIPTYIRRHHGQEPVSYAEFPHA
EKYLRPILDETYGIPVYQEQIMQIASQVAGYSLGEADLLRRAMGKKRVEE
MQKHRERFVRGAKERGVPEEEANRLFDMLEAFANYGFNKSHAAAYSLLSY
QTAYVKAHYPVEFMAALLSVERHDSDKVAEYIRDARALGIPVLPPDVNRS
GFDFKVVGEEILFGLSAVKNVGEMAARAILEERERGGPFKSLGDFLKRLP
EQVVNKRALESLVKAGALDAFGDRARLLASLEPLLRWAAETRERGRSGLV
GLFAEVEEPPLVEASPLDEITMLRYEKEALGIYVSGHPVLRYPGLREVAS
CTIEELSEFVRELPGKPKVLLSGMVEEVRFTLSDETGALEVVKEDIPLLV
LAEVERVLAQAVWTLEEVLEAPKALEVEVDHALLDEKGARLKSLLDEHPG
SLPVYLRVLGPFGEALFALREVRVGEEALGLLEAEGYRAYLVPDREVF

3D structure

• PDB: 3e0d Insights into the Replisome from the Structure of a Ternary Complex of the DNA Polymerase III alpha-Subunit.
• Chain: A
• Resolution: 4.6 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	K233 D470 R473 R509 F529 R570 H571 S573 A576 D595 L620 G621 L622 R623 T624 S672	K224 D444 R447 R483 F503 R544 H545 S547 A550 D569 L594 G595 L596 R597 T598 S646
BS02	dna	A	R424 A499 K501 A502 K531 H575 M606 D618	R398 A473 K475 A476 K505 H549 M580 D592
BS03	DTP	A	R424 G425 S426 D465 H817	R398 G399 S400 D439 H791

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0008408 3'-5' exonuclease activity

Biological Process

• GO:0006260 DNA replication
• GO:0071897 DNA biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3e0d, PDBe:3e0d, PDBj:3e0d
• PDBsum: 3e0d
• PubMed: 18691598
• UniProt: Q9XDH5|DPO3A_THEAQ DNA polymerase III subunit alpha (Gene Name=dnaE)