Structure of PDB 3df0 Chain A

Receptor sequence
>3df0A (length=676) Species: 10116 (Rattus norvegicus) [Search protein sequence]
AIKYLNQDYETLRNECLEAGALFQDPSFPALPSSLGFKELGPYSSKTRGI
EWKRPTEICADPQFIIGGATRTDICQGALGDSWLLAAIASLTLNEEILAR
VVPLDQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEG
SEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELRKPPPN
LFKIIQKALEKGSLLGCSIDITSASEAVTYQKLVKGHAYSVTGAEEVESS
GSLQKLIRIRNPWGQVEWTGKWNDNCPSWNTVDPEVRANLTERQEDGEFW
MSFSDFLRHYSRLEICNLTPDTLTCDSYKKWKLTKMDGNWRRGSTAGGCR
NYPNTFWMNPQYLIKLEEEDEDDEDGERGCTFLVGLIQKHRRRQRKMGED
MHTIGFGIYEVPEELTGQTNIHLSKNFFLTTRARERSDTFINLREVLNRF
KLPPGEYVLVPSTFEPHKNGDFCIRVFSEKKADYQTVDDEIEANIEEIEA
NEEDIGDGFRRLFAQLAGEDAEISAFELQTILRRVLAKREDIKSDGFSIE
TCKIMVDMLDEDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSY
EMRKALEEAGFKLPCQLHQVIVARFADDELIIDFDNFVRCLVRLEILFKI
FKQLDPENTGTIQLDLISWLSFSVLG
3D structure
PDB3df0 Concerted multi-pronged attack by calpastatin to occlude the catalytic cleft of heterodimeric calpains.
ChainA
Resolution2.95 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Q99 S105 H262 N286 W288
Catalytic site (residue number reindexed from 1) Q76 S82 H237 N261 W263
Enzyme Commision number 3.4.22.53: calpain-2.
Interaction with ligand
Gene Ontology
Molecular Function
GO:0004198 calcium-dependent cysteine-type endopeptidase activity
GO:0005509 calcium ion binding
GO:0005515 protein binding
GO:0008092 cytoskeletal protein binding
GO:0008233 peptidase activity
GO:0008234 cysteine-type peptidase activity
GO:0019899 enzyme binding
GO:0044877 protein-containing complex binding
GO:0046872 metal ion binding
Biological Process
GO:0001666 response to hypoxia
GO:0001824 blastocyst development
GO:0006508 proteolysis
GO:0007520 myoblast fusion
GO:0007565 female pregnancy
GO:0009612 response to mechanical stimulus
GO:0010666 positive regulation of cardiac muscle cell apoptotic process
GO:0016540 protein autoprocessing
GO:0030163 protein catabolic process
GO:0032675 regulation of interleukin-6 production
GO:0035458 cellular response to interferon-beta
GO:0042542 response to hydrogen peroxide
GO:0048266 behavioral response to pain
GO:0048488 synaptic vesicle endocytosis
GO:0051603 proteolysis involved in protein catabolic process
GO:0071222 cellular response to lipopolysaccharide
GO:0071230 cellular response to amino acid stimulus
GO:0140249 protein catabolic process at postsynapse
GO:1901741 positive regulation of myoblast fusion
GO:2001247 positive regulation of phosphatidylcholine biosynthetic process
Cellular Component
GO:0000785 chromatin
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005764 lysosome
GO:0005783 endoplasmic reticulum
GO:0005794 Golgi apparatus
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0005925 focal adhesion
GO:0009897 external side of plasma membrane
GO:0030425 dendrite
GO:0031143 pseudopodium
GO:0042995 cell projection
GO:0043025 neuronal cell body
GO:0045121 membrane raft
GO:0097038 perinuclear endoplasmic reticulum
GO:0098793 presynapse
GO:0098794 postsynapse
GO:0110158 calpain complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3df0, PDBe:3df0, PDBj:3df0
PDBsum3df0
PubMed19020622
UniProtQ07009|CAN2_RAT Calpain-2 catalytic subunit (Gene Name=Capn2)

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