Structure of PDB 3d2h Chain A

Receptor sequence

>3d2hA (length=498) Species: 3467 (Eschscholzia californica)

DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAII
LPGSKEELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNL
NRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHI
SGGGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGG
GVWGAIYAWKIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEE
DFTLSVLGGADEKQVWLTMLGFHFGLKTVAKSTFDLLFPELGLVEEDYLE
MSWGESFAYLAGLETVSQLNNRFLKFDERAFKTKVDLTKEPLPSKAFYGL
LERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRSGTRLMVEYIVAWNQS
EQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVV
NNAIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMANFD

3D structure

• PDB: 3d2h A concerted mechanism for berberine bridge enzyme
• Chain: A
• Resolution: 1.65 Å

Enzymatic activity

• Enzyme Commision number: 1.21.3.3: reticuline oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	L99 R100 S101 G102 G103 H104 S105 Y106 S110 S141 G164 W165 C166 V169 G170 G172 G173 H174 F180 G225 G226 A230 I231 Y456 N458	L74 R75 S76 G77 G78 H79 S80 Y81 S85 S116 G139 W140 C141 V144 G145 G147 G148 H149 F155 G200 G201 A205 I206 Y431 N433
BS02	FSH	A	L99 R100 S101 G102 G103 H104 S105 Y106 S110 S141 G164 C166 V169 G170 G172 G173 H174 F180 G225 G226 A230 I231 N458	L74 R75 S76 G77 G78 H79 S80 Y81 S85 S116 G139 C141 V144 G145 G147 G148 H149 F155 G200 G201 A205 I206 N433

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0050468 reticuline oxidase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0071949 FAD binding

Biological Process

• GO:0009820 alkaloid metabolic process

Cellular Component

• GO:0031410 cytoplasmic vesicle

External links

• PDB: RCSB:3d2h, PDBe:3d2h, PDBj:3d2h
• PDBsum: 3d2h
• PubMed: 18953357
• UniProt: P30986|RETO_ESCCA Reticuline oxidase (Gene Name=BBE1)