Structure of PDB 3cv9 Chain A

Receptor sequence
>3cv9A (length=401) Species: 1909 (Streptomyces griseolus) [Search protein sequence]
PQTTDAPAFPSNRSCPYQLPDGYAQLRDTPGPLHRVTLYDGRQAWVVTKH
EAARKLLGDPRLSSNATDDNFPATSPAFEAVRESPQAFIGLDPPEHGTRR
RMTISEFTVKRIKGMRPEVEEVVHGFLDEMLAAGPTADLVSQFALPVPSM
VICRLLGVPYADHEFFQDASKRLVQSTDAQSALTARNDLAGYLDGLITQF
QTEPGAGLVGALVADQLANGEIDREELISTAMLLLIAGHETTASMTSLSV
ITLLDHPEQYAALRADRSLVPGAVEELLRYLAIADIAGGRVATADIEVEG
QLIRAGEGVIVVNSIANRDGTVYEDPDALDIHRSARHHLAFGFGVHQCLG
QNLARLELEVILNALMDRVPTLRLAVPVEQLVLRPGTTIQGVNELPVTWH
H
3D structure
PDB3cv9 Structure-based design of a highly active vitamin D hydroxylase from Streptomyces griseolus CYP105A1
ChainA
Resolution1.7 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Q182 A244 E247 T248 T249 A291 C355 L356 G357 E364 I396
Catalytic site (residue number reindexed from 1) Q175 A237 E240 T241 T242 A284 C348 L349 G350 E357 I389
Enzyme Commision number 1.14.15.22: vitamin D 1,25-hydroxylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEM A F95 I96 H103 R107 L241 A244 G245 T248 T249 A294 R297 A347 F348 H353 C355 G357 F88 I89 H96 R100 L234 A237 G238 T241 T242 A287 R290 A340 F341 H346 C348 G350
BS02 VDX A T81 F85 L180 R193 I293 G296 T395 T74 F78 L173 R186 I286 G289 T388
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
GO:0020037 heme binding
GO:0046872 metal ion binding
Biological Process
GO:0070640 vitamin D3 metabolic process
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3cv9, PDBe:3cv9, PDBj:3cv9
PDBsum3cv9
PubMed18937506
UniProtP18326|CPXE_STRGO Vitamin D3 dihydroxylase (Gene Name=cyp105A1)

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