Structure of PDB 3cv8 Chain A

Receptor sequence
>3cv8A (length=402) Species: 1909 (Streptomyces griseolus) [Search protein sequence]
TPQTTDAPAFPSNRSCPYQLPDGYAQLRDTPGPLHRVTLYDGRQAWVVTK
HEAARKLLGDPRLSSNRTDDNFPATSPFFEAVRESPQAFIGLDPPEHGTR
RRMTISEFTVKRIKGMRPEVEEVVHGFLDEMLAAGPTADLVSQFALPVPS
MVICRLLGVPYADHEFFQDASKRLVQSTDAQSALTARNDLAGYLDGLITQ
FQTEPGAGLVGALVADQLANGEIDREELISTAMLLLIAGHETTASMTSLS
VITLLDHPEQYAALRADRSLVPGAVEELLRYLAIADIAGGRVATADIEVE
GQLIRAGEGVIVVNSIANRDGTVYEDPDALDIHRSARHHLAFGFGVHQCL
GQNLARLELEVILNALMDRVPTLRLAVPVEQLVLRPGTTIQGVNELPVTW
HH
3D structure
PDB3cv8 Structure-based design of a highly active vitamin D hydroxylase from Streptomyces griseolus CYP105A1
ChainA
Resolution2.0 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Q182 A244 E247 T248 T249 A291 C355 L356 G357 E364 I396
Catalytic site (residue number reindexed from 1) Q176 A238 E241 T242 T243 A285 C349 L350 G351 E358 I390
Enzyme Commision number 1.14.15.22: vitamin D 1,25-hydroxylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEM A F95 I96 H103 R107 F114 L240 L241 A244 T248 T249 A294 R297 A347 F348 H353 C355 L356 G357 F89 I90 H97 R101 F108 L234 L235 A238 T242 T243 A288 R291 A341 F342 H347 C349 L350 G351
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
GO:0020037 heme binding
GO:0046872 metal ion binding
Biological Process
GO:0070640 vitamin D3 metabolic process
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3cv8, PDBe:3cv8, PDBj:3cv8
PDBsum3cv8
PubMed18937506
UniProtP18326|CPXE_STRGO Vitamin D3 dihydroxylase (Gene Name=cyp105A1)

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