BioLiP

PDB

3csi The anti-cancer drug chlorambucil as a substrate for the human polymorphic enzyme glutathione transferase P1-1: kinetic properties and crystallographic characterisation of allelic variants.

Chain

Resolution

1.9 Å

3D
structure

Catalytic site residues are labeled in the structure
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Site
#

Ligand

Ligand
chain

Binding residues on receptor
(original residue number in PDB)

Binding residues on receptor
(residue number reindexed from 1)

Binding affinity

BS01

LZ6

F8 P9 V10 R13 V35 W38 K44 Q51 L52 Q64 S65 Y108 G205

BS02

GSH

F8 R13 W38 Q51 L52 Q64 S65

Molecular Function

GO:0004364

glutathione transferase activity

GO:0004602

glutathione peroxidase activity

fatty acid binding

protein binding

JUN kinase binding

transferase activity

kinase regulator activity

GO:0035730

S-nitrosoglutathione binding

GO:0035731

dinitrosyl-iron complex binding

GO:0070026

nitric oxide binding

	Biological Process
GO:0000302	response to reactive oxygen species
GO:0002674	negative regulation of acute inflammatory response
GO:0006469	negative regulation of protein kinase activity
GO:0006629	lipid metabolic process
GO:0006693	prostaglandin metabolic process
GO:0006749	glutathione metabolic process
GO:0006805	xenobiotic metabolic process
GO:0007417	central nervous system development
GO:0009890	negative regulation of biosynthetic process
GO:0010804	negative regulation of tumor necrosis factor-mediated signaling pathway
GO:0032691	negative regulation of interleukin-1 beta production
GO:0032720	negative regulation of tumor necrosis factor production
GO:0032872	regulation of stress-activated MAPK cascade
GO:0032873	negative regulation of stress-activated MAPK cascade
GO:0032930	positive regulation of superoxide anion generation
GO:0035726	common myeloid progenitor cell proliferation
GO:0035732	nitric oxide storage
GO:0043066	negative regulation of apoptotic process
GO:0043124	negative regulation of canonical NF-kappaB signal transduction
GO:0043407	negative regulation of MAP kinase activity
GO:0043409	negative regulation of MAPK cascade
GO:0043508	negative regulation of JUN kinase activity
GO:0043651	linoleic acid metabolic process
GO:0048147	negative regulation of fibroblast proliferation
GO:0051122	hepoxilin biosynthetic process
GO:0051771	negative regulation of nitric-oxide synthase biosynthetic process
GO:0070372	regulation of ERK1 and ERK2 cascade
GO:0070373	negative regulation of ERK1 and ERK2 cascade
GO:0070664	negative regulation of leukocyte proliferation
GO:0071222	cellular response to lipopolysaccharide
GO:0071638	negative regulation of monocyte chemotactic protein-1 production
GO:0098869	cellular oxidant detoxification
GO:1901687	glutathione derivative biosynthetic process
GO:2001237	negative regulation of extrinsic apoptotic signaling pathway

Biological Process

GO:0000302

response to reactive oxygen species

GO:0002674

negative regulation of acute inflammatory response

GO:0006469

negative regulation of protein kinase activity

GO:0006629

lipid metabolic process

GO:0006693

prostaglandin metabolic process

GO:0006749

glutathione metabolic process

GO:0006805

xenobiotic metabolic process

GO:0007417

central nervous system development

GO:0009890

negative regulation of biosynthetic process

GO:0010804

negative regulation of tumor necrosis factor-mediated signaling pathway

GO:0032691

negative regulation of interleukin-1 beta production

GO:0032720

negative regulation of tumor necrosis factor production

GO:0032872

regulation of stress-activated MAPK cascade

GO:0032873

negative regulation of stress-activated MAPK cascade

GO:0032930

positive regulation of superoxide anion generation

GO:0035726

common myeloid progenitor cell proliferation

GO:0035732

nitric oxide storage

GO:0043066

negative regulation of apoptotic process

GO:0043124

negative regulation of canonical NF-kappaB signal transduction

GO:0043407

negative regulation of MAP kinase activity

GO:0043409

negative regulation of MAPK cascade

GO:0043508

negative regulation of JUN kinase activity

GO:0043651

linoleic acid metabolic process

GO:0048147

negative regulation of fibroblast proliferation

GO:0051122

hepoxilin biosynthetic process

GO:0051771

negative regulation of nitric-oxide synthase biosynthetic process

GO:0070372

regulation of ERK1 and ERK2 cascade

GO:0070373

negative regulation of ERK1 and ERK2 cascade

GO:0070664

negative regulation of leukocyte proliferation

GO:0071222

cellular response to lipopolysaccharide

GO:0071638

negative regulation of monocyte chemotactic protein-1 production

GO:0098869

cellular oxidant detoxification

GO:1901687

glutathione derivative biosynthetic process

GO:2001237

negative regulation of extrinsic apoptotic signaling pathway

	Cellular Component
GO:0005576	extracellular region
GO:0005615	extracellular space
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005829	cytosol
GO:0031982	vesicle
GO:0034774	secretory granule lumen
GO:0070062	extracellular exosome
GO:0097057	TRAF2-GSTP1 complex
GO:1904813	ficolin-1-rich granule lumen

Cellular Component

extracellular region

extracellular space

nucleus

cytoplasm

mitochondrion