Structure of PDB 3c5g Chain A

Receptor sequence

>3c5gA (length=324) Species: 9606 (Homo sapiens)

ATNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQE
ACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKT
AQMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQT
VQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLL
DSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSE
FACALLYFTGSAHFNRSMKALAKTKGMSLSEHALSTAVVHGCKVGPGRVL
PTPTEKDVFRLLGLPYREPAERDW

3D structure

• PDB: 3c5g Substrate-induced DNA strand misalignment during catalytic cycling by DNA polymerase lambda.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D179 D181 D242
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 V462 S463 K472 R514 K517 L527 E529 H530	W26 V214 S215 K224 R266 K269 L279 E281 H282
BS02	dna	A	W342 G343 A344 G345 K347 T348 D490 Y505	W94 G95 A96 G97 K99 T100 D242 Y257
BS03	dna	A	Y267 W274 R275 Y279 P303 G304 G306 R308 M309 K312	Y19 W26 R27 Y31 P55 G56 G58 R60 M61 K64
BS04	dna	A	V537 G548 R549	V289 G297 R298
BS05	MG	A	D427 D429	D179 D181
BS06	D3T	A	R386 S417 R420 G426 D427 D429 Y505 A510 N513	R138 S169 R172 G178 D179 D181 Y257 A262 N265

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3c5g, PDBe:3c5g, PDBj:3c5g
• PDBsum: 3c5g
• PubMed: 18369368
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)