Structure of PDB 3c0p Chain A

Receptor sequence

>3c0pA (length=496) Species: 4577 (Zea mays)

PWPASLAALALDGKLRTDSNATAAASTDFGNITSALPAAVLYPSSTADLV
ALLSAANSTPGWPYTIAFRGRGHSLMGQAFAPGGVVVNMASLGDAAAPPR
INVSADGRYVDAGGEQVWIDVLRASLARGVAPRSWTDYLYLTVGGTLSNA
GISGQAFRHGPQISNVLEMDVITGHGEMVTCSKQLNADLFDAVLGGLGQF
GVITRARIAVEPAPARARWVRLVYTDFAAFSADQERLTAPRSFGPMSYVE
GSVFVNQSLATDLANTGFFTDADVARIVALAGERNATTVYSIEATLNYDN
ATAAAAAVDQELASVLGTLSYVEGFAFQRDVAYAAFLDRVHGEEVALNKL
GLWRVPHPWLNMFVPRSRIADFDRGVFKGILQGTDIVGPLIVYPLNKSMW
DDGMSAATPSEDVFYAVSLLFSSVAPNDLARLQEQNRRILRFCDLAGIQY
KTYLARHTDRSDWVRHFGAAKWNRFVEMKNKYDPKRLLSPGQDIFN

3D structure

• PDB: 3c0p Mechanism-based inhibitors of cytokinin oxidase/dehydrogenase attack FAD cofactor
• Chain: A
• Resolution: 1.95 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H105 D169 E288
• Catalytic site (residue number reindexed from 1): H73 D137 E250
• Enzyme Commision number: 1.5.99.12: cytokinin dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	F61 F100 G102 R103 G104 H105 S106 Q110 T168 D169 Y170 L173 T174 G176 G177 T178 S180 N181 I184 V234 I235 W391 Y491 L492 S527	F29 F68 G70 R71 G72 H73 S74 Q78 T136 D137 Y138 L141 T142 G144 G145 T146 S148 N149 I152 V202 I203 W353 Y453 L454 S489
BS02	HA8	A	D169 V378 E381 L458	D137 V340 E343 L420

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0016491 oxidoreductase activity
• GO:0019139 cytokinin dehydrogenase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0071949 FAD binding

Biological Process

• GO:0009690 cytokinin metabolic process

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:3c0p, PDBe:3c0p, PDBj:3c0p
• PDBsum: 3c0p
• PubMed: 18571199
• UniProt: Q9T0N8|CKX1_MAIZE Cytokinin dehydrogenase 1 (Gene Name=CKX1)