Structure of PDB 3bw7 Chain A

Receptor sequence

>3bw7A (length=497) Species: 4577 (Zea mays)

RPWPASLAALALDGKLRTDSNATAAASTDFGNITSALPAAVLYPSSTADL
VALLSAANSTPGWPYTIAFRGRGHSLMGQAFAPGGVVVNMASLGDAAAPP
RINVSADGRYVDAGGEQVWIDVLRASLARGVAPRSWTDYLYLTVGGTLSN
AGISGQAFRHGPQISNVLEMDVITGHGEMVTCSKQLNADLFDAVLGGLGQ
FGVITRARIAVEPAPARARWVRLVYTDFAAFSADQERLTAPRSFGPMSYV
EGSVFVNQSLATDLANTGFFTDADVARIVALAGERNATTVYSIEATLNYD
NATAAAAAVDQELASVLGTLSYVEGFAFQRDVAYAAFLDRVHGEEVALNK
LGLWRVPHPWLNMFVPRSRIADFDRGVFKGILQGTDIVGPLIVYPLNKSM
WDDGMSAATPSEDVFYAVSLLFSSVAPNDLARLQEQNRRILRFCDLAGIQ
YKTYLARHTDRSDWVRHFGAAKWNRFVEMKNKYDPKRLLSPGQDIFN

3D structure

• PDB: 3bw7 Mechanism-based inhibitors of cytokinin oxidase/dehydrogenase attack FAD cofactor
• Chain: A
• Resolution: 1.95 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H105 D169 E288
• Catalytic site (residue number reindexed from 1): H74 D138 E251
• Enzyme Commision number: 1.5.99.12: cytokinin dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	F61 F100 G102 R103 G104 H105 S106 Q110 T168 D169 Y170 L173 T174 G176 G177 S180 N181 I184 V234 I235 W391 Y491 L492 S527	F30 F69 G71 R72 G73 H74 S75 Q79 T137 D138 Y139 L142 T143 G145 G146 S149 N150 I153 V203 I204 W354 Y454 L455 S490
BS02	301	A	D169 V378 W397 L458 L492	D138 V341 W360 L421 L455

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0016491 oxidoreductase activity
• GO:0019139 cytokinin dehydrogenase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0071949 FAD binding

Biological Process

• GO:0009690 cytokinin metabolic process

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:3bw7, PDBe:3bw7, PDBj:3bw7
• PDBsum: 3bw7
• PubMed: 18571199
• UniProt: Q9T0N8|CKX1_MAIZE Cytokinin dehydrogenase 1 (Gene Name=CKX1)