Structure of PDB 3bul Chain A

Receptor sequence

>3bulA (length=577) Species: 562 (Escherichia coli)

QAEWRSWEVNKRLEYSLVKGITEFIEQDTEEARQQATRPCEVIEGPLMDG
MNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASKEQCKTNGKMV
IATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNADL
IGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNY
SGPTVYVQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPR
TPPVTLEAARDNDFAFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPF
FMTWSLAGKYPRILEDEVVGVEAQRLFKDANDMLDKLSAEKTLNPRGVVG
LFPANRVGDDIEIYRDETRTHVINVSHHLRQQTEKTGFANYCLADFVAPK
LSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMVKALADRLAEAFA
EYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHTEKAT
IWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQV
EDYARRKGMSVTEVERWLAPNLGYDAD

3D structure

• PDB: 3bul A disulfide-stabilized conformer of methionine synthase reveals an unexpected role for the histidine ligand of the cobalamin cofactor.
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D757 H759 S810
• Catalytic site (residue number reindexed from 1): D107 H109 S160
• Enzyme Commision number: 2.1.1.13: methionine synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	B12	A	H759 V766 G802 L803 S804 L806 I807 T808 G833 G834 Q858 N859 A860 T863 M952 D1093 A1136 P1137 G1138 Y1139 H1145 K1148 M1171 G1174 V1177 S1178	H109 V116 G152 L153 S154 L156 I157 T158 G183 G184 Q208 N209 A210 T213 M302 D443 A486 P487 G488 Y489 H495 K498 M521 G524 V527 S528

Gene Ontology

Molecular Function

• GO:0008705 methionine synthase activity
• GO:0031419 cobalamin binding
• GO:0046872 metal ion binding

Biological Process

• GO:0009086 methionine biosynthetic process

External links

• PDB: RCSB:3bul, PDBe:3bul, PDBj:3bul
• PDBsum: 3bul
• PubMed: 18332423
• UniProt: P13009|METH_ECOLI Methionine synthase (Gene Name=metH)