Structure of PDB 3bk2 Chain A

Receptor sequence

>3bk2A (length=549) Species: 262724 (Thermus thermophilus HB27)

QDHVEIIPLGGMGEIGKNITVFRFRDEIFVLDGGLAFPEEGMPGVDLLIP
RVDYLIEHRHKIKAWVLTHGHEDHIGGLPFLLPMIFGKESPVPIYGARLT
LGLLRGKLEEFGLRPGAFNLKEISPDDRIQVGRYFTLDLFRMTHSIPDNS
GVVIRTPIGTIVHTGDFKLDPTPIDGKVSHLAKVAQAGAEGVLLLIADAT
NAERPGYTPSEMEIAKELDRVIGRAPGRVFVTTFASHIHRIQSVIWAAEK
YGRKVAMEGRSMLKFSRIALELGYLKVKDRLYTLEEVKDLPDHQVLILAT
GSQGQPMSVLHRLAFEGHAKMAIKPGDTVILSSSPIPGNEEAVNRVINRL
YALGAYVLYPPTYKVHASGHASQEELKLILNLTTPRFFLPWHGEVRHQMN
FKWLAESMSRPPEKTLIGENGAVYRLTRETFEKVGEVPHGVLYVDGLGVG
DITEEILADRRHMAEEGLVVITALAGEDPVVEVVSRGFVKAGERLLGEVR
RMALEALKNGVREKKPLERIRDDIYYPVKKFLKKATGRDPMILPVVIEG

3D structure

• PDB: 3bk2 Structural insights into the dual activity of RNase J
• Chain: A
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	H75 H77 H150 D172	H69 H71 H144 D166
BS02	ZN	A	D79 H80 D172 H398	D73 H74 D166 H392
BS03	U5P	A	D79 F240 S340 P341 I342 P343 H372 S374	D73 F234 S334 P335 I336 P337 H366 S368

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0004534 5'-3' RNA exonuclease activity
• GO:0008270 zinc ion binding
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006364 rRNA processing
• GO:0006396 RNA processing

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3bk2, PDBe:3bk2, PDBj:3bk2
• PDBsum: 3bk2
• PubMed: 18204464
• UniProt: Q72JJ7|RNJ_THET2 Ribonuclease J (Gene Name=rnj)