Structure of PDB 3b97 Chain A

Receptor sequence
>3b97A (length=432) Species: 9606 (Homo sapiens) [Search protein sequence]
SILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRD
NDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTE
NKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFN
VINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYG
KDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEF
FRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDW
GAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVT
ESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRS
ERLAKYNQLLRIEEELGSKAKFAGRNFRNPLA
3D structure
PDB3b97 Structure of human alpha-enolase (hENO1), a multifunctional glycolytic enzyme.
ChainA
Resolution2.2 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) S39 H157 E166 E209 D244 E292 D317 K342 H370 K393
Catalytic site (residue number reindexed from 1) S39 H157 E166 E209 D244 E292 D317 K342 H370 K393
Enzyme Commision number 4.2.1.11: phosphopyruvate hydratase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A D244 E292 D317 D244 E292 D317
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding
GO:0001222 transcription corepressor binding
GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific
GO:0003677 DNA binding
GO:0003714 transcription corepressor activity
GO:0003723 RNA binding
GO:0004634 phosphopyruvate hydratase activity
GO:0005515 protein binding
GO:0016829 lyase activity
GO:0042803 protein homodimerization activity
GO:0045296 cadherin binding
GO:0046872 metal ion binding
GO:0051020 GTPase binding
Biological Process
GO:0000122 negative regulation of transcription by RNA polymerase II
GO:0006094 gluconeogenesis
GO:0006096 glycolytic process
GO:0009615 response to virus
GO:0010756 positive regulation of plasminogen activation
GO:0030308 negative regulation of cell growth
GO:0045892 negative regulation of DNA-templated transcription
GO:0045933 positive regulation of muscle contraction
GO:0061621 canonical glycolysis
GO:1903298 negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
GO:2001171 positive regulation of ATP biosynthetic process
Cellular Component
GO:0000015 phosphopyruvate hydratase complex
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005640 nuclear outer membrane
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0005938 cell cortex
GO:0009986 cell surface
GO:0016020 membrane
GO:0031430 M band
GO:0070062 extracellular exosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3b97, PDBe:3b97, PDBj:3b97
PDBsum3b97
PubMed18560153
UniProtP06733|ENOA_HUMAN Alpha-enolase (Gene Name=ENO1)

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