Structure of PDB 3b3r Chain A

Receptor sequence

>3b3rA (length=503) Species: 74576 (Streptomyces sp. SA-COO)

SADNGGYVPAVVIGTGYGAAVSALRLGEAGVQTLMLEMGQLWNQPGPDGN
IFCGMLNPDKRSSWFKNRTEAPLGSFLWLDVVNRNIDPYAGVLDRVNYDQ
MSVYVGRGVGGGSLVNGGMAVEPKRSYFEEILPRVDSSEMYDRYFPRANS
MLRVNHIDTKWFEDTEWYKFARVSREQAGKAGLGTVFVPNVYDFGYMQRE
AAGEVPKSALATEVIYGNNHGKQSLDKTYLAAALGTGKVTIQTLHQVKTI
RQTKDGGYALTVEQKDTDGKLLATKEISCRYLFLGAGSLGSTELLVRARD
TGTLPNLNSEVGAGWGPNGNIMTARANHMWNPTGAHQSSIPALGIDAWDN
SDSSVFAQIAPMPAGLETWVSLYLAITKNPQRGTFVYDAATDRAKLNWTR
DQNAPAVNAAKALFDRINKANGTIYRYDLFGTQLKAFADDFCYQPLGGCV
LGKATDDYGRVAGYKNLYVTDGSLIPGSVGVNPFVTITALAERNVERIIK
QDV

3D structure

• PDB: 3b3r Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase
• Chain: A
• Resolution: 0.98 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Q361 Q447 N485
• Catalytic site (residue number reindexed from 1): Q358 Q444 N482
• Enzyme Commision number: 1.1.3.6: cholesterol oxidase.
  5.3.3.1: steroid Delta-isomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAE	A	I16 G19 Y20 L39 E40 M41 Y107 R110 G114 G115 N119 G120 M122 Q249 V250 A289 G290 Y446 D474 G475 N485 P486 F487	I13 G16 Y17 L36 E37 M38 Y104 R107 G111 G112 N116 G117 M119 Q246 V247 A286 G287 Y443 D471 G472 N482 P483 F484
BS02	GOL	A	G120 P344 Y446	G117 P341 Y443
BS03	GOL	A	L44 L237	L41 L234

Gene Ontology

Molecular Function

• GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
• GO:0050660 flavin adenine dinucleotide binding

External links

• PDB: RCSB:3b3r, PDBe:3b3r, PDBj:3b3r
• PDBsum: 3b3r
• PubMed: 18029419
• UniProt: P12676|CHOD_STRS0 Cholesterol oxidase (Gene Name=choA)