Structure of PDB 3ay0 Chain A

Receptor sequence
>3ay0A (length=320) Species: 2190 (Methanocaldococcus jannaschii) [Search protein sequence]
PLCLKINKKHGEQTRRILIENNLLNKDYKITSEGNYLYLPIKDVDEDILK
SILNIEFELVDKELEEKSFREIISKKYRKEIDEGLISLSYDVVGDLVILQ
ISDEVDEKIRKEIGELAYKLIPCKGVFRRVRELEHLAGENRTLTIHKENG
YRLWVDIAKVYFSPRLGGERARIMKKVSLNDVVVDMFAGVGPFSIACKNA
KKIYAIDINPHAIELLKKNIKLNKLEHKIIPILSDVREVDVKGNRVIMNL
PKFAHKFIDKALDIVEEGGVIHYYTIGKDFDKAIKLFEKKCDCEVLEKRI
VKSYAPREYILALDFKINKK
3D structure
PDB3ay0 Differentiating analogous tRNA methyltransferases by fragments of the methyl donor.
ChainA
Resolution3.05 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.228: tRNA (guanine(37)-N(1))-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A K175 H227 E230 K159 H211 E214
BS02 ADN A Y177 F203 D223 I224 D251 V252 F273 Y161 F187 D207 I208 D235 V236 F257 PDBbind-CN: -logKd/Ki=3.04,Kd=910uM
Gene Ontology
Molecular Function
GO:0008168 methyltransferase activity
GO:0008175 tRNA methyltransferase activity
GO:0016740 transferase activity
GO:0052906 tRNA (guanine(37)-N1)-methyltransferase activity
Biological Process
GO:0002939 tRNA N1-guanine methylation
GO:0008033 tRNA processing
GO:0030488 tRNA methylation
GO:0032259 methylation
Cellular Component
GO:0005737 cytoplasm

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Biological Process
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Cellular Component
External links
PDB RCSB:3ay0, PDBe:3ay0, PDBj:3ay0
PDBsum3ay0
PubMed21602303
UniProtQ58293|TRM5B_METJA tRNA (guanine(37)-N1)-methyltransferase Trm5b (Gene Name=trm5b)

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