Structure of PDB 3aw9 Chain A

Receptor sequence

>3aw9A (length=304) Species: 410359 (Pyrobaculum calidifontis JCM 11548)

MRIVVTGGAGFIGSHLVDKLVELGYEVVVVDIVQRDTGGSAELHVRDLKD
YSWGAGIKGDVVFHFAANPEVRTTEPIVHFNENVVATFNVLEWARQTGVR
TVVFASSSTVYGDADVIPTPEEEPYKPISVYGAAKAAGEVMCATYARLFG
VRCLAVRYANVVGPRLRHGVIYDFIMKLRRNPNVLEVLGDGTQRKSYLYV
RDAVEATLAAWKKFEEMDAPFLALNVGNVDAVRVLDIAQIVAEVLGLRPE
IRLVPSTPDGRGWPGDVKYMTLAVTKLMKLTGWRPTMTSAEAVKKTAEDL
AKEL

3D structure

• PDB: 3aw9 Structure of UDP-galactose 4-epimerase mutant
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): A107 S109 S110 T111 Y133 K137 L168 G171
• Catalytic site (residue number reindexed from 1): A105 S107 S108 T109 Y131 K135 L166 G169

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GDU	A	P69 V71 S109 Y133 N162 H170 V172 D175 L190 Q195 K197 Y199 V236 W265 D268	P69 V71 S107 Y131 N160 H168 V170 D173 L188 Q193 K195 Y197 V234 W263 D266
BS02	NAD	A	G7 G10 F11 I12 D31 I32 R46 D47 L48 F65 A66 A67 E84 A107 S108 Y133 K137 Y160 N162 V163 H170	G7 G10 F11 I12 D31 I32 R46 D47 L48 F65 A66 A67 E82 A105 S106 Y131 K135 Y158 N160 V161 H168

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003978 UDP-glucose 4-epimerase activity
• GO:0016491 oxidoreductase activity

Biological Process

• GO:0033499 galactose catabolic process via UDP-galactose

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:3aw9, PDBe:3aw9, PDBj:3aw9
• PDBsum: 3aw9
• UniProt: A3MUJ4