Structure of PDB 3amr Chain A

Receptor sequence

>3amrA (length=352) Species: 1423 (Bacillus subtilis)

LSDPYHFTVNAAAETEPVDTAGDAADDPAIWLDPKTPQNSKLITTNKKSG
LVVYSLDGKMLHSYNTGKLNNVDIRYDFPLNGKKVDIAAASNRSEGKNTI
EIYAIDGKNGTLQSMTDPDHPIATAINEVYGFTLYHSQKTGKYYAMVTGK
EGEFEQYELKADKNGYISGKKVRAFKMNSQTEGMAADDEYGRLYIAEEDE
AIWKFSAEPDGGSNGTVIDRADGRHLTRDIEGLTIYYAADGKGYLMASSQ
GNSSYAIYDRQGKNKYVADFRITDGPETDGTSDTDGIDVLGFGLGPEYPF
GIFVAQDGENIDHGQKANQNFKIVPWERIADQIGFRPLANEQVDPRKLTD
RS

3D structure

• PDB: 3amr Crystal structures of Bacillus alkaline phytase in complex with divalent metal ions and inositol hexasulfate
• Chain: A
• Resolution: 1.25 Å

Enzymatic activity

• Enzyme Commision number: 3.1.3.8: 3-phytase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	IHS	A	D24 K48 K49 R94 Y131 Q251 D286	D23 K47 K48 R93 Y130 Q250 D285
BS02	CA	A	E15 D280 N311 I312 D313	E14 D279 N310 I311 D312
BS03	CA	A	D220 H226	D219 H225
BS04	CA	A	D200 D230	D199 D229
BS05	CA	A	D28 P29 V73	D27 P28 V72
BS06	CA	A	D27 E183 D286	D26 E182 D285
BS07	CA	A	D24 D286	D23 D285
BS08	CA	A	Y131 E183 E199 E232	Y130 E182 E198 E231
BS09	CA	A	D230 E232 Q251	D229 E231 Q250
BS10	CA	A	D280 G281 D308 E310	D279 G280 D307 E309

Gene Ontology

Molecular Function

• GO:0016158 3-phytase activity
• GO:0016787 hydrolase activity

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:3amr, PDBe:3amr, PDBj:3amr
• PDBsum: 3amr
• PubMed: 21463636
• UniProt: O31097|PHYC_BACIU 3-phytase (Gene Name=phyC)