Structure of PDB 3akg Chain A

Receptor sequence

>3akgA (length=447) Species: 227882 (Streptomyces avermitilis MA-4680 = NBRC 14893)

TFTNPLAEKRADPHIFKHTDGYYYFTATVPEYDRIVLRRATTLQGLATAP
ETTIWTKHASGVMGAHIWAPEIHFIDGKWYVYFAAGSTSDVWAIRMYVLE
SGAANPLTGSWTEKGQIATPVSSFSLDATTFVVNGVRHLAWAQRNPAEDN
NTSLFIAKMANPWTISGTPTEISQPTLSWETVGYKVNEGPAVIQHGGKVF
LTYSASATDANYCLGMLSASASADLLNAASWTKSSQPVFKTSEATGQYGP
GHNSFTVSEDGKSDILVYHDRNYKDISGDPLNDPNRRTRLQKVYWNADGT
PNFGIPVADGVTPVRFSSYNYPDRYIRHWDFRARIEANVTNLADSQFRVV
TGLAGSGTISLESANYPGYYLRHKNYEVWVEKNDGSSAFKNDASFSRRAG
LADSADGIAFESYNYPGRYLRHYENLLRIQPVSTALDRQDATFYAEK

3D structure

• PDB: 3akg Crystal Structure of an Exo-1,5-{alpha}-L-arabinofuranosidase from Streptomyces avermitilis Provides Insights into the Mechanism of Substrate Discrimination between Exo- and Endo-type Enzymes in Glycoside Hydrolase Family 43.
• Chain: A
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.55: non-reducing end alpha-L-arabinofuranosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	AHR	A	H336 W337 D338 F339 D352	H328 W329 D330 F331 D344
BS02	AHR	A	N159 V194 E196	N151 V186 E188
BS03	AHR	A	H430 Y431 E432 N433 D448	H422 Y423 E424 N425 D440

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0046556 alpha-L-arabinofuranosidase activity

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0031222 arabinan catabolic process
• GO:0046373 L-arabinose metabolic process

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:3akg, PDBe:3akg, PDBj:3akg
• PDBsum: 3akg
• PubMed: 20739278
• UniProt: Q82P90|IABF_STRAW Extracellular exo-alpha-(1->5)-L-arabinofuranosidase (Gene Name=Araf43A)