Structure of PDB 3air Chain A

Receptor sequence
>3airA (length=491) Species: 4565 (Triticum aestivum) [Search protein sequence]
GPVFTKLKPWQIPKRDWFDKDFLFGASTSAYQIEGAWNEDGKGPSTWDHF
CHTYPERISDMTNGDVAANSYHLYEEDVKALKDMGMKVYRFSISWSRILP
DGTGKVNQAGIDYYNKLINSLIDNDIVPYVTIWHWDTPQALEDKYGGFLN
RQIVDDYKQFAEVCFKNFGDRVKNWFTFNEPHTYCCFSYGEGIHAPGRCS
PGMDCAVPEGDSLREPYTAGHHILLAHAEAVQLFKARYNMHGDSKIGMAF
DVMGYEPYQDSFLDDQARERSIDYNMGWFLEPVVRGDYPFSMRSLIGDRL
PMFTKEEQEKLASSCDIMGLNYYTSRFSKHVDMSPDFTPTLNTDDAYASS
ETTGSDGNDIGPITGTYWIYMYPKGLTDLLLIMKEKYGNPPVFITENGIA
DVEGDESMPDPLDDWKRLDYLQRHISAVKDAIDQGADVRGHFTWGLIDNF
EWSLGYSSRFGLVYIDKNDGNKRKLKKSAKWFSKFNSVPKP
3D structure
PDB3air Active-site architecture of benzoxazinone-glucoside beta-D-glucosidases in Triticeae
ChainA
Resolution2.0 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) E45 C62 R101 H145 D166 D167 A172 E191 T194 N332 Y334 E407
Catalytic site (residue number reindexed from 1) E34 C51 R90 H134 D155 D156 A161 E180 T183 N321 Y323 E396
Enzyme Commision number 3.2.1.182: 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase.
3.2.1.21: beta-glucosidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 G2F A Q43 H145 E191 Y334 E407 W455 E462 W463 F471 Q32 H134 E180 Y323 E396 W444 E451 W452 F460
BS02 DNF A W146 E191 T194 F198 W463 W135 E180 T183 F187 W452
Gene Ontology
Molecular Function
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0008422 beta-glucosidase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0102726 DIMBOA glucoside beta-D-glucosidase activity
Biological Process
GO:0005975 carbohydrate metabolic process
Cellular Component
GO:0009507 chloroplast

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3air, PDBe:3air, PDBj:3air
PDBsum3air
PubMed21421370
UniProtQ1XH05|HGL1B_WHEAT 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1b, chloroplastic (Gene Name=GLU1B)

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