Structure of PDB 3afl Chain A

Receptor sequence

>3aflA (length=766) Species: 176299 (Agrobacterium fabrum str. C58)

QTLLDEPRPGSLTIGYEPSEEAQPTENPPRFSWLPDIDDGARYVLRISTD
PGFTDKKTLVFEDLAWNFFTPDEALPDGHYHWCYALWDQKSATAHSNWST
VRSFEISEALPKTPLPGRSARHAAAQTSHPRLWLNSEQLSAFADAVAKDP
NHCGWAEFYEKSVEPWLERPVMPEPQPYPNNTRVATLWRQMYIDCQEVIY
AIRHLAIAGRVLGRDDLLDASRKWLLAVAAWDTKGATSRAYNDEAGFRVV
VALAWGYDWLYDHLSEDERRTVRSVLLERTREVADHVIAHARIHVFPYDS
HAVRSLSAVLTPACIALQGESDEAGEWLDYTVEFLATLYSPWAGTDGGWA
EGPHYWMTGMAYLIEAANLIRSYIGYDLYQRPFFQNTGRFPLYTKAPGTR
RANFGDDSTLGDLPGLKLGYNVRQFAGVTGNGHYQWYFDHIKADATGTEM
AFYNYGWWDLNFDDLVYRHDYPQVEAVSPADLPALAVFDDIGWATIQKDM
EDPDRHLQFVFKSSPYGSLSASHGDQNAFVLYAHGEDLAIQSGYYVAFNS
QMHLNWRRQTRSKNAVLIGGKGQYAEKDKALARRAAGRIVSVEEQPGHVR
IVGDATAAYQVANPLVQKVLRETHFVNDSYFVIVDEVECSEPQELQWLCH
TLGAPQTGRSSFRYNGRKAGFYGQFVYSSGGTPQISAVEGFPDIDPKEFE
GLDIHHHVCATVPAATRHRLVTLLVPYSLKEPKRIFSFIDDQGFSTDIYF
SDVDDERFKLSLPKQF

3D structure

• PDB: 3afl Crystal structure of exotype alginate lyase Atu3025 from Agrobacterium tumefaciens
• Chain: A
• Resolution: 2.99 Å

Enzymatic activity

• Enzyme Commision number: 4.2.2.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	LGU	A	S310 H311 F558	S300 H301 F548
BS02	LGU	A	W198 H311 R314 Y365 F558	W188 H301 R304 Y355 F548
BS03	MAW	A	W198 R199 Y202 H364 S418 F462 W467 Y555	W188 R189 Y192 H354 S408 F452 W457 Y545

Gene Ontology

Molecular Function

• GO:0016829 lyase activity
• GO:0047757 chondroitin-glucuronate 5-epimerase activity

Biological Process

• GO:0030204 chondroitin sulfate metabolic process
• GO:0030205 dermatan sulfate metabolic process

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:3afl, PDBe:3afl, PDBj:3afl
• PDBsum: 3afl
• PubMed: 20507980
• UniProt: A9CEJ9