Structure of PDB 3af6 Chain A

Receptor sequence

>3af6A (length=637) Species: 70601 (Pyrococcus horikoshii OT3)

TQVDQILRDIRAVVNQMVPKEAKITEIEFEGPELVIYVKNPEELIKDLAK
VLKKRISVRPDPEVLLPPEEAEKLIFEIVPKEAEITNIAFDPSVGEVLIE
AKKPGLVIGKNGETLRLITQKVKWAPKVVRTPPLQSQTIYSIRQILQTES
KDRRKFLRQVGRNIYRKPEYKSRWIRITGLGGFREVGRSALLVQTDESFV
LVDFGVNVAMLNDPYKAFPHFDAPEFQYVLREGLLDAIIITHAHLDHCGM
LPYLFRYNLFDGPIYTTPPTRDLMVLLQKDFIEIQQSNGQDPLYRPRDIK
EVIKHTITLDYGEVRDISPDIRLTLHNAGHILGSAIVHLHIGNGLHNIAI
TGDFKFIPTRLLEPANAKFPRLETLVMESTYGGANDIQMPREEAEKRLIE
VIHNTIKRGGKVLIPAMAVGRAQEVMMVLEEYARIGGIEVPIYLDGMIWE
ATAIHTAYPEYLSRRLREQIFKEGYNPFLSEIFHPVANSRERQDIIDSNE
PAIIIASSGMLVGGPSVEYFKQLAPDPKNSIIFVSYQAEGTLGRQVQSGI
REIPMVGEEGRTEVIKVNMEVHTIDGFSGHADRRELMNYVAKVRPRPERI
ITVHGEPQKCLDLATSIHRKFGISTRAPNNLDTIRLR

3D structure

• PDB: 3af6 Crystal structure of an archaeal cleavage and polyadenylation specificity factor subunit from Pyrococcus horikoshii
• Chain: A
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	I59 K60 A63 K67 K68 R69 I70 V328 R329 D330 R336 H354 G358 N361 P384 R385	I45 K46 A49 K53 K54 R55 I56 V314 R315 D316 R322 H340 G344 N347 P370 R371
BS02	rna	A	H632 T639	H618 T625
BS03	ZN	A	H256 H258 H344	H242 H244 H330
BS04	ZN	A	D260 H261 D367 H618	D246 H247 D353 H604

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006353 DNA-templated transcription termination

External links

• PDB: RCSB:3af6, PDBe:3af6, PDBj:3af6
• PDBsum: 3af6
• PubMed: 20544974
• UniProt: O50112|FTTA_PYRHO Transcription termination factor FttA (Gene Name=fttA)