Structure of PDB 3a4a Chain A

Receptor sequence
>3a4aA (length=586) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
SSAHPETEPKWWKEATFYQIYPASFKDSNDDGWGDMKGIASKLEYIKELG
ADAIWISPFYDSPQDDMGYDIANYEKVWPTYGTNEDCFALIEKTHKLGMK
FITDLVINHCSSEHEWFKESRSSKTNPKRDWFFWRPPKGYDAEGKPIPPN
NWKSYFGGSAWTFDEKTQEFYLRLFCSTQPDLNWENEDCRKAIYESAVGY
WLDHGVDGFRIDVGSLYSKVVGLPDAPVVDKNSTWQSSDPYTLNGPRIHE
FHQEMNQFIRNRVKDGREIMTVGEMQHASDETKRLYTSASRHELSELFNF
SHTDVGTSPLFRYNLVPFELKDWKIALAELFRYINGTDCWSTIYLENHDQ
PRSITRFGDDSPKNRVISGKLLSVLLSALTGTLYVYQGQELGQINFKNWP
VEKYEDVEIRNNYNAIKEEHGENSEEMKKFLEAIALISRDHARTPMQWSR
EEPNAGFSGPSAKPWFYLNDSFREGINVEDEIKDPNSVLNFWKEALKFRK
AHKDITVYGYDFEFIDLDNKKLFSFTKKYNNKTLFAALNFSSDATDFKIP
NDDSSFKLEFGNYPKKEVDASSRTLKPWEGRIYISE
3D structure
PDB3a4a Crystal structures of isomaltase from Saccharomyces cerevisiae and in complex with its competitive inhibitor maltose
ChainA
Resolution1.6 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D107 D215 E277 H351 D352
Catalytic site (residue number reindexed from 1) D104 D212 E274 H348 D349
Enzyme Commision number 3.2.1.10: oligo-1,6-glucosidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GLC A D69 Y72 H112 F178 D215 V216 E277 H351 D352 R442 D66 Y69 H109 F175 D212 V213 E274 H348 D349 R439
BS02 CA A D30 N32 D34 W36 D38 D27 N29 D31 W33 D35
Gene Ontology
Molecular Function
GO:0004556 alpha-amylase activity
GO:0004558 alpha-1,4-glucosidase activity
GO:0004574 oligo-1,6-glucosidase activity
GO:0004575 sucrose alpha-glucosidase activity
GO:0005515 protein binding
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0033934 glucan 1,4-alpha-maltotriohydrolase activity
GO:0090599 alpha-glucosidase activity
Biological Process
GO:0000023 maltose metabolic process
GO:0000025 maltose catabolic process
GO:0005975 carbohydrate metabolic process
GO:0005987 sucrose catabolic process
GO:0046352 disaccharide catabolic process
Cellular Component
GO:0005739 mitochondrion

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3a4a, PDBe:3a4a, PDBj:3a4a
PDBsum3a4a
PubMed20812985
UniProtP53051|MALX3_YEAST Oligo-1,6-glucosidase IMA1 (Gene Name=IMA1)

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