Structure of PDB 2zxe Chain A

Receptor sequence

>2zxeA (length=992) Species: 7797 (Squalus acanthias)

LDELKKEVSMDDHKLSLDELHNKYGTDLTRGLTNARAKEILARDGPNSLT
PPPTTPEWIKFCRQLFGGFSILLWIGAILCFLAYGIQAATEDEPANDNLY
LGVVLSTVVIVTGCFSYYQEAKSSRIMDSFKNMVPQQALVIRDGEKSTIN
AEFVVAGDLVEVKGGDRIPADLRIISAHGCKVDNSSLTGESEPQTRSPEF
SSENPLETRNIAFFSTNCVEGTARGVVVYTGDRTVMGRIATLASGLEVGR
TPIAIEIEHFIHIITGVAVFLGVSFFILSLILGYSWLEAVIFLIGIIVAN
VPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTL
TQNRMTVAHMWFDNQIHEADTTENQSGAAFDKTSATWSALSRIAALCNRA
VFQAGQDNVPILKRSVAGDASESALLKCIELCCGSVQGMRDRNPKIVEIP
FNSTNKYQLSIHENEKSSESRYLLVMKGAPERILDRCSTILLNGAEEPLK
EDMKEAFQNAYLELGGLGERVLGFCHFALPEDKYNEGYPFDADEPNFPTT
DLCFVGLMAMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGV
GIISEGNETIEDIAARLNIPIGQVNPRDAKACVVHGSDLKDLSTEVLDDI
LHYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIG
VAMGISGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLT
SNIPEITPFLVFIIGNVPLPLGTVTILCIDLGTDMVPAISLAYEQAESDI
MKRQPRNPKTDKLVNERLISMAYGQIGMIQALGGFFSYFVILAENGFLPM
DLIGKRVRWDDRWISDVEDSFGQQWTYEQRKIVEFTCHTSFFISIVVVQW
ADLIICKTRRNSIFQQGMKNKILIFGLFEETALAAFLSYCPGTDVALRMY
PLKPSWWFCAFPYSLIIFLYDEMRRFIIRRSPGGWVEQETYY

3D structure

• PDB: 2zxe Crystal structure of the sodium - potassium pump at 2.4 A resolution
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D376 D717 D721
• Catalytic site (residue number reindexed from 1): D345 D686 D690

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MF4	A	T219 E221 D376 K377 T378 T617 K698	T188 E190 D345 K346 T347 T586 K667
BS02	MG	A	D376 T378 D717	D345 T347 D686
BS03	K	A	P333 T779 S782 N783 E786 D811	P302 T748 S751 N752 E755 D780
BS04	K	A	V329 A330 N331 V332 P333 E334 N783 E786 D811	V298 A299 N300 V301 P302 E303 N752 E755 D780
BS05	K	A	L725 K726 K727 A728 D729 I730 G731 D747	L694 K695 K696 A697 D698 I699 G700 D716
BS06	CLR	A	E847 S851	E816 S820

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0005215 transporter activity
• GO:0005391 P-type sodium:potassium-exchanging transporter activity
• GO:0005524 ATP binding
• GO:0008556 P-type potassium transmembrane transporter activity
• GO:0016887 ATP hydrolysis activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006813 potassium ion transport
• GO:0006814 sodium ion transport
• GO:0006883 intracellular sodium ion homeostasis
• GO:0010248 establishment or maintenance of transmembrane electrochemical gradient
• GO:0030007 intracellular potassium ion homeostasis
• GO:0036376 sodium ion export across plasma membrane
• GO:1902600 proton transmembrane transport
• GO:1990573 potassium ion import across plasma membrane

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:2zxe, PDBe:2zxe, PDBj:2zxe
• PDBsum: 2zxe
• PubMed: 19458722
• UniProt: Q4H132