Structure of PDB 2zxc Chain A

Receptor sequence

>2zxcA (length=643) Species: 287 (Pseudomonas aeruginosa)

DLPYRFGLGKADITGEAAEVGMMGYSSLEQKTAGIHMRQWARAFVIEEAA
SGRRLVYVNTDLGMIFQAVHLKVLARLKAKYPGVYDENNVMLAATHTHSG
PGGFSHYAMYNLSVLGFQEKTFNAIVDGIVRSIERAQARLQPGRLFYGSG
ELRNASRNRSLLSHLKNPDIAGYEDGIDPQMSVLSFVDANGELAGAISWF
PVHSTSMTNANHLISPDNKGYASYHWEHDVSRKSGFVAAFAQTNAGNLSP
NLNLKPGSGPFDNEFDNTREIGLRQFAKAYEIAGQAQEEVLGELDSRFRF
VDFTRLPIRPEFTDGQPRQLCTAAIGTSLAAGSTEDGPGPLGLEEGNNPF
LSALGGLLTGVPPQELVQCQAEKTILADTGNKKPYPWTPTVLPIQMFRIG
QLELLGAPAEFTVMAGVRIRRAVQAASEAAGIRHVVFNGYANAYASYVTT
REEYAAQEYEGGSTLYGPWTQAAYQQLFVDMAVALRERLPVETSAIAPDL
SCCQMNFQTGVVADDPYIGKSFGDVLQQPRESYRIGDKVTVAFVTGHPKN
DLRTEKTFLEVVNIGKDGKQTPVTVATDNDWDTQYRWERVGISASKATIS
WSIPPGTEPGHYYIRHYGNAKNFWTQKISEIGGSTRSFEVLGT

3D structure

• PDB: 2zxc Mechanistic insights into the hydrolysis and synthesis of ceramide by neutral ceramidase.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 3.5.1.23: ceramidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	H97 H204 E411 Y448	H96 H203 E410 Y447
BS02	2ED	A	Y26 H204 S334 T335 G338 Y460	Y25 H203 S333 T334 G337 Y459
BS03	MG	A	H37 D579 N580 D581 T584	H36 D578 N579 D580 T583

Gene Ontology

Molecular Function

• GO:0016787 hydrolase activity
• GO:0017040 N-acylsphingosine amidohydrolase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006629 lipid metabolic process
• GO:0042759 long-chain fatty acid biosynthetic process
• GO:0046512 sphingosine biosynthetic process
• GO:0046514 ceramide catabolic process
• GO:0051872 sphingosine catabolic process

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:2zxc, PDBe:2zxc, PDBj:2zxc
• PDBsum: 2zxc
• PubMed: 19088069
• UniProt: Q9I596|NCASE_PSEAE Neutral ceramidase (Gene Name=PA0845)