Structure of PDB 2zwo Chain A

Receptor sequence

>2zwoA (length=395) Species: 311400 (Thermococcus kodakarensis)

NTIRVIVSVDKAKFNPHEVLGIGGHIVYQFKLIPAVVVDVPANAVGKLKK
MPGVEKVEFDHQAVLLGKPSWLGGGSTQPAQTIPWGIERVKAPSVWSITD
GSVSVIQVAVLDTGVDYDHPDLAANIAWCVSTLRGKVSTKLRDCADQNGH
GTHVIGTIAALNNDIGVVGVAPGVQIYSVRVLDARGSGSYSDIAIGIEQA
ILGPDGVADKDGDGIIAGDPDDAAAEVISMSLGGPADDSYLYDMIIQAYN
AGIVIVAASGNEGAPSPSYPAAYPEVIAVGAIDSNDNIASFSNRQPEVSA
PGVDILSTYPDDSYETLMGTAMATPHVSGVVALIQAAYYQKYGKILPVGT
FDDISKNTVRGILHITADDLGPTGWDADYGYGVVRAALAVQAALG

3D structure

• PDB: 2zwo Requirement of a unique Ca(2+)-binding loop for folding of Tk-subtilisin from a hyperthermophilic archaeon.
• Chain: A
• Resolution: 2.07 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D115 H153 N264 A324
• Catalytic site (residue number reindexed from 1): D112 H150 N261 A321
• Enzyme Commision number: 3.4.21.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	Q84 D124 L164 N166 I168 V170	Q81 D121 L161 N163 I165 V167
BS02	CA	A	D212 D214 D216 I218 D222 D225	D209 D211 D213 I215 D219 D222
BS03	CA	A	D214 D216 D222 D224	D211 D213 D219 D221
BS04	CA	A	V108 Q110 A227 E229	V105 Q107 A224 E226
BS05	CA	A	D119 D121 D314 D315	D116 D118 D311 D312
BS06	CA	A	D372 L373 P375 G377 D379	D369 L370 P372 G374 D376

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0008236 serine-type peptidase activity
• GO:0042802 identical protein binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:2zwo, PDBe:2zwo, PDBj:2zwo
• PDBsum: 2zwo
• PubMed: 19813760
• UniProt: P58502|TKSU_THEKO Tk-subtilisin (Gene Name=TK1675)