Structure of PDB 2zwa Chain A

Receptor sequence
>2zwaA (length=672) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
QERRKKYADLAIQGTNNSSIASKRSVELLYLPKLSSANNFQMDKNNKLLE
YFKFFVPKKIKRSPCINRGYWLRLFAIRSRLNSIIEQTPQDKKIVVVNLG
CGYDPLPFQLLDTNNIQSQQYHDRVSFIDIDYSDLLKIKIELIKTIPELS
KIIGLSEDKDYVDDSNVDFLTTPKYLARPCDLNDSKMFSTLLNECQLYDP
NVVKVFVAEVSLAYMKPERSDSIIEATSKMENSHFIILEQLIPKGPFEPF
SKQMLAHFKRNDSPLQSVLKYNTIESQVQRFNKLGFAYVNVGDMFQLWES
ADEATKKELLKVEPFDELEEFHLFCHHYVLCHATNYKEFAFTQGFLFDRI
NLTVDEDYQLLECECPINRKFGDVDVAGNDVFYMGGSNPYRVNEILQLSI
HYDKIDMKNIEVSSSEVPVARMCHTFTTISRNNQLLLIGGRKAPHQGLSD
NWIFDMKTREWSMIKSLSHTRFRHSACSLPDGNVLILGGVTEGPAMLLYN
VTEEIFKDVTPKDEFFQNSLVSAGLEFDPVSKQGIILGGGFMDQTTVSDK
AIIFKYDAENATEPITVIKKLQHPLFQRYGSQIKYITPRKLLIVGGTSPS
GLFDRTNSIISLDPLSETLTSIPISRRIWEDHSLMLAGFSLVSTTIHIIG
GGATCYGFGSVTNVGLKLIAIA
3D structure
PDB2zwa Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4.
ChainA
Resolution1.7 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.290: tRNA(Phe) [7-(3-amino-3-carboxypropyl)wyosine(37)-O]-methyltransferase.
2.3.1.231: tRNA(Phe) {7-[3-amino-3-(methoxycarbonyl)propyl]wyosine(37)-N}- methoxycarbonyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SAH A I27 Q28 T30 N31 S34 K38 R88 G115 C116 D146 Y147 C195 D196 L197 E224 S226 I12 Q13 T15 N16 S19 K23 R73 G100 C101 D131 Y132 C180 D181 L182 E209 S211
Gene Ontology
Molecular Function
GO:0008168 methyltransferase activity
GO:0008175 tRNA methyltransferase activity
Biological Process
GO:0006400 tRNA modification
GO:0008033 tRNA processing
GO:0030488 tRNA methylation
GO:0031591 wybutosine biosynthetic process
GO:0032259 methylation
Cellular Component
GO:0005737 cytoplasm
GO:0005739 mitochondrion

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2zwa, PDBe:2zwa, PDBj:2zwa
PDBsum2zwa
PubMed19287006
UniProtQ08282|TYW4_YEAST tRNA wybutosine-synthesizing protein 4 (Gene Name=PPM2)

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