Structure of PDB 2zux Chain A

Receptor sequence

>2zuxA (length=582) Species: 1423 (Bacillus subtilis) [Search protein sequence]

ARQMEALNRGLVAVKTDGGIFVSWRFLGTENASVLFNVYRDGQKLNAAPV
KTTNYVDKNGSAGSTYTVRAVVNGTEQPASEKASVWAQPYHSVPLDKPAG
GTTPKGESYTYSANDASVGDVDGDGQYELILKWDPSNSKDNSQDGYTGDV
LIDAYKLDGTKLWRINLGKNIRAGAHYTQFMVYDLDGDGKAEVAMKTADG
TKDGTGKVIGNANADYRNEQGRVLSGPEYLTVFQGSTGKELVTANFEPAR
GNVSDWGDSYGNRVDRFLAGIAYLDGQRPSLIMTRGYYAKTMLVAYNFRD
GKLSKLWTLDSSKSGNEAFAGQGNHNLSIADVDGDGKDEIIFGSMAVDHD
GKGMYSTGLGHGDALHTGDLDPGRPGLEVFQVHEDKNAKYGLSFRDAATG
KILWGVYAGKDVGRGMAADIDPRYPGQEVWANGSLYSAKGVKIGSGVPSS
TNFGIWWDGDLLREQLDSNRIDKWDYQNGVSKNMLTASGAAANNGTKATP
TLQADLLGDWREEVVWRTEDSSALRIYTTTIPTEHRLYTLMHDPVYRLGI
AWQNIAYNQPPHTSFFLGDGMAEQPKPNMYTP

3D structure

PDB

2zux Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases

Chain

Resolution

1.32 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

4.2.2.23: rhamnogalacturonan endolyase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	CA	A	H363 H399 D401 E422	H325 H361 D363 E384
BS02	CA	A	D153 N592 A594 N596	D115 N554 A556 N558
BS03	CA	A	D543 L545 G546 D547 R549 E551	D505 L507 G508 D509 R511 E513
BS04	CA	A	D496 D498 L500 E502	D458 D460 L462 E464
BS05	CA	A	D222 D224 D226 K228 E230	D184 D186 D188 K190 E192
BS06	CA	A	D158 D160 D162 Q164 E166	D120 D122 D124 Q126 E128
BS07	CA	A	D457 D459 Y462 G464 E466	D419 D421 Y424 G426 E428
BS08	CA	A	D407 D409 R412 G414 E416	D369 D371 R374 G376 E378
BS09	CA	A	D371 D373 E377 D386 H387	D333 D335 E339 D348 H349
BS10	CA	A	D369 D371 D373 K375 E377	D331 D333 D335 K337 E339
BS11	RAM	A	N532 G533 T534 K535 Y595	N494 G495 T496 K497 Y557
BS12	RAM	A	D172 N532 G533	D134 N494 G495
BS13	RAM	A	P142 D187 K207 R255	P104 D149 K169 R217
BS14	RAM	A	G238 K240	G200 K202

Gene Ontology

	Molecular Function
GO:0016829	lyase activity
GO:0046872	metal ion binding
GO:0102210	rhamnogalacturonan endolyase activity

	Biological Process
GO:0071555	cell wall organization

	Cellular Component
GO:0005576	extracellular region

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:2zux, PDBe:2zux, PDBj:2zux
PDBsum	2zux
PubMed	19193638
UniProt	O31526\|YESW_BACSU Rhamnogalacturonan endolyase YesW (Gene Name=yesW)

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