Structure of PDB 2zj5 Chain A

Receptor sequence

>2zj5A (length=680) Species: 2261 (Pyrococcus furiosus)

MRVDELRVDERIKSTLKERGIESFYPPQAEALKSGILEGKNALISIPTAS
GKTLIAEIAMVHRILTQGGKAVYIVPLKALAEEKFQEFQDWEKIGLRVAM
ATGDYDSKDEWLGKYDIIIATAEKFDSLLRHGSSWIKDVKILVADEIHLI
GSRDRGATLEVILAHMLGKAQIIGLSATIGNPEELAEWLNAELIVSDWRP
VKLRRGVFYQGFVTWEDGSIDRFSSWEELVYDAIRKKKGALIFVNMRRKA
ERVALELSKKVKSLLTKPEIRALNELADSLEENPTNEKLAKAIRGGVAFH
HAGLGRDERVLVEENFRKGIIKAVVATPTLSPAFRVIIRDIWRYSDFGME
RIPIIEVHQMLGRAGRPKYDEVGEGIIVSTSDDPREVMNHYIFGKPEKLF
SQLSNESNLRSQVLALIATFGYSTVEEILKFISNTFYAYQRKDTYSLEEK
IRNILYFLLENEFIEISLEDKIRPLSLGIRTAKLYIDPYTAKMFKDKMEE
VVKDPNPIGIFHLISLTPDITPFNYSKREFERLEEEYYEFKDRLYFDDPY
ERKFFRAFKTALVLLAWINEVPEGEIVEKYSVEPGDIYRIVETAEWLVYS
LKEIAKVLGAYEIVDYLETLRVRVKYGIREELIPLMQLPLVGRRRARALY
NSGFRSIEDISQARPEELLKIEVEAIFKFL

3D structure

• PDB: 2zj5 Atomic structures and functional implications of the archaeal RecQ-like helicase Hjm
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.4: DNA 3'-5' helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	A	S23 Y25 Q28 T48 G51 K52 T53 L54	S23 Y25 Q28 T48 G51 K52 T53 L54

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016818 hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
• GO:0016853 isomerase activity
• GO:0016887 ATP hydrolysis activity
• GO:0043138 3'-5' DNA helicase activity

Biological Process

• GO:0006281 DNA repair
• GO:0032508 DNA duplex unwinding

External links

• PDB: RCSB:2zj5, PDBe:2zj5, PDBj:2zj5
• PDBsum: 2zj5
• PubMed: 19159486
• UniProt: O73946|HELS_PYRFU ATP-dependent DNA helicase Hel308 (Gene Name=hel308)