Structure of PDB 2zio Chain A

Receptor sequence
>2zioA (length=255) Species: 2209 (Methanosarcina mazei) [Search protein sequence]
PALTKSQTDRLEVLLNPKDESGKPFRELESELLSRRKKDLQQIYAEEREN
YLGKLEREITRFFVDRGFLEIKSPILIPLEYIERMGIDNLSKQIFRVDKN
FCLRPMLAPNLYNYLRKLDRALPDPIKIFEIGPCYRKESDGEHLEEFTML
NFCQMGSGCTRENLESIITDFLNHLGIDFKIVGDYGDTLDVMHGDLELSS
AVVGPIPLDREWGIDKPWIGAGFGLERLLKVKHDFKNIKRAARSGSYYNG
ISTNL
3D structure
PDB2zio Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode N(varepsilon)-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification
ChainA
Resolution2.06 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 6.1.1.26: pyrrolysine--tRNA(Pyl) ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 2PN A H338 E396 R426 H143 E197 R227
BS02 AYB A A302 R330 E337 L339 F342 M344 N346 E396 L397 S399 V401 W417 A420 G421 G423 R426 A108 R136 E142 L144 F147 M149 N151 E197 L198 S200 V202 W218 A221 G222 G224 R227
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0005524 ATP binding
Biological Process
GO:0043039 tRNA aminoacylation

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Molecular Function
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Biological Process
External links
PDB RCSB:2zio, PDBe:2zio, PDBj:2zio
PDBsum2zio
PubMed19022179
UniProtQ8PWY1|PYLS_METMA Pyrrolysine--tRNA ligase (Gene Name=pylS)

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