Structure of PDB 2yfs Chain A

Receptor sequence

>2yfsA (length=533) Species: 33959 (Lactobacillus johnsonii)

KAELKGQVKDIVEESGVDTSKLTNDQINELNKINFSKEAKSGTQLTYNDF
KKIAKTLIEQDARYAIPFFNASKIKNMPAAKTLDAQSGKVEDLEIWNSWP
VQDAKTGYVSNWNGYQLVIGMMGVPNVNDNHIYLLYNKYGDNDFNHWKNA
GPIFGLGTPVIQQWSGSATLNKDGSIQLYYTKVDTSDNNTNHQKLASATV
YLNLEKDQDKISIAHVDNDHIVFEGDGYHYQTYDQWKETNKGADNIAMRD
AHVIDDDNGNRYLVFEASTGTENYQGDDQIYQWLNYGGTNKDNLGDFFQI
LSNSDIKDRAKWSNAAIGIIKLNDDVKNPSVAKVYSPLISAPMVSDEIER
PDVVKLGNKYYLFAATRLNRGSNDDAWMATNKAVGDNVAMIGYVSDNLTH
GYVPLNESGVVLTASVPANWRTATYSYYAVPVEGRDDQLLITSYITNRGE
VAGKGMHATWAPSFLLQINPDNTTTVLAKMTNQGDWIWDDSSENPDMMGV
LEKDAPNSAALPGEWGKPVDWDLIGGYNLKPHQ

3D structure

• PDB: 2yfs Crystal Structure of Inulosucrase from Lactobacillus: Insights Into the Substrate Specificity and Product Specificity of Gh68 Fructansucrases.
• Chain: A
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N272 S340 D425 E524
• Catalytic site (residue number reindexed from 1): N97 S165 D250 E349
• Enzyme Commision number: 2.4.1.9: inulosucrase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	E522 E524 R542	E347 E349 R367
BS02	FRU	A	W271 N272 M296 S340 D425 E524	W96 N97 M121 S165 D250 E349
BS03	FRU	A	N301 V302 R623	N126 V127 R448
BS04	CA	A	D419 Q450 W487 N489 D521	D244 Q275 W312 N314 D346
BS05	CA	A	N317 D660 I662 S667	N142 D485 I487 S492

Gene Ontology

Molecular Function

• GO:0050053 levansucrase activity

Biological Process

• GO:0009758 carbohydrate utilization

External links

• PDB: RCSB:2yfs, PDBe:2yfs, PDBj:2yfs
• PDBsum: 2yfs
• PubMed: 21801732
• UniProt: Q74K42|INUS_LACJO Inulosucrase (Gene Name=inuJ)