Structure of PDB 2ya1 Chain A

Receptor sequence

>2ya1A (length=981) Species: 170187 (Streptococcus pneumoniae TIGR4)

AHMASDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSFKDAK
KDDYGYYLDVKLKEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLD
QDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGT
DFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLK
NHSQIFLKDDDESIYTNPYYVHDIRMTGAQHESSFSTLILKHSNITNIDE
AGKKVTTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQV
DLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLG
ITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDSKIDDAHKVAKAA
FVDPAKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPF
GTFEAFIEKLDYLKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSN
YNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLINEIHKRGMGAILDVV
YNHTAKVDLFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMTKRLLIDS
IKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTY
AGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGK
RDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPS
KAENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRNPAYRTPVA
EDKVPNKSHLLRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYP
ENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDRVHLITVPGQNGVEKEDV
VIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEVLADENQAGP
VGIANPKGLEWTEKGLKLNALTATVLRVSQN

3D structure

• PDB: 2ya1 The Conformation and Function of a Multimodular Glycogen-Degrading Pneumococcal Virulence Factor.
• Chain: A
• Resolution: 2.25 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): W545 S640 Y641 T676 D778 E807 D895
• Catalytic site (residue number reindexed from 1): W383 S478 Y479 T514 D616 E645 D733
• Enzyme Commision number: 3.2.1.41: pullulanase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	A	Y667 H715 F743 D778	Y505 H553 F581 D616
BS02	GLC	A	D989 Y991	D827 Y829
BS03	GLC	A	W156 W168 K969	W27 W39 K807
BS04	GLC	A	W156 W158 L202 D214 F743	W27 W29 L72 D84 F581
BS05	GLC	A	W809 D839 R842 Y849	W647 D677 R680 Y687
BS06	GLC	A	Y849 N896	Y687 N734
BS07	GLC	A	K969 D989	K807 D827
BS08	GLC	A	W263 W276 K318	W133 W146 K188
BS09	GLC	A	W263 W265 N323	W133 W135 N193

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0030246 carbohydrate binding

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:2ya1, PDBe:2ya1, PDBj:2ya1
• PDBsum: 2ya1
• PubMed: 21565699
• UniProt: A0A0H2UNG0|PULA_STRPN Pullulanase A (Gene Name=spuA)