Structure of PDB 2y7h Chain A

Receptor sequence

>2y7hA (length=464) Species: 562 (Escherichia coli) [Search protein sequence]

MSAGKLPEGWVIAPVSTVTTLIRGVTYKKEQAINYLKDDYLPLIRANNIQ
NGKFDTTDLVFVPKNLVKESQKISPEDIVIAMSSGSKSVVGKSAHQHLPF
ECSFGAFCGVLRPEKLIFSGFIAHFTKSSLYRNKISSLSAGANINNIKPA
SFDLINIPIPPLAEQKIIAEKLDTLLAQVDSTKARFEQIPQILKRFRQAV
LGGAVNGKLTEKWRNFEPQHSVFKKLNFESILTELRNGLSSKPNESGVGH
PILRISSVRAGHVDQNDIRFLECSESELNRHKLQDGDLLFTRYNGSLEFV
GVCGLLKKLQHQNLLYPDKLIRARLTKDALPEYIEIFFSSPSARNAMMNC
VKTTSGQKGISGKDIKSQVVLLPPVKEQAEIVRRVEQLFAYADTIEKQVN
NALARVNNLTQSILAKAFRGELTAQWRAENPDLISGENSAAALLEKIKAE
RAASGGKKASRKKS

3D structure

PDB

2y7h The Structure of M.Ecoki Type I DNA Methyltransferase with a DNA Mimic Antirestriction Protein.

Chain

Resolution

18.0 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.21.3: type I site-specific deoxyribonuclease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R23 G24 S70 Q71 K72 N143 N145 R254 I255 R259 Y293 N294 K319 K358	R23 G24 S70 Q71 K72 N143 N145 R254 I255 R259 Y293 N294 K319 K358
BS02	dna	A	Y27 E30 A46 Q50 S84 G85 F104 N145 E234 R236 N279 R280 H281 R292 Y293	Y27 E30 A46 Q50 S84 G85 F104 N145 E234 R236 N279 R280 H281 R292 Y293

Gene Ontology

	Molecular Function
GO:0003677	DNA binding

	Biological Process
GO:0009307	DNA restriction-modification system

	Cellular Component
GO:0019812	type I site-specific deoxyribonuclease complex

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Cellular Component

External links

PDB	RCSB:2y7h, PDBe:2y7h, PDBj:2y7h
PDBsum	2y7h
PubMed	19074193
UniProt	P05719\|T1SK_ECOLI Type I restriction enzyme EcoKI specificity subunit (Gene Name=hsdS)

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