Structure of PDB 2xrz Chain A

Receptor sequence

>2xrzA (length=450) Species: 192952 (Methanosarcina mazei Go1)

HMNPKRIRALKSGKQGDGPVVYWMSRDQRAEDNWALLFSRAIAKEANVPV
VVVFCLTDEFLEAGIRQYEFMLKGLQELEVSLSRKKIPSFFLRGDPGEKI
SRFVKDYNAGTLVTDFSPLRIKNQWIEKVISGISIPFFEVDAHNVVPCWE
ASQKHEYAAHTFRPKLYALLPEFLEEFPELEPNSVETLSDVLETGVKALL
PERALLKNKDPLFEPWHFEPGEKAAKKVMESFIADRLDSYGALRNDPTKN
MLSNLSPYLHFGQISSQRVVLEVEKAESNPGSKKAFLDEILIWKEISDNF
CYYNPGYDGFESFPSWAKESLNAHRNDVRSHIYTLEEFEAGKTHDPLWNA
SQMELLSTGKMHGYTRMYWAKKILEWSESPEKALEIAICLNDRYELDGRD
PNGYAGIAWSIGGVHDRAWGEREVTGKIRYMSYEGCKRKFDVKLYIEKYS

3D structure

• PDB: 2xrz Crystal Structures of an Archaeal Class II DNA Photolyase and its Complex with Uv-Damaged Duplex DNA.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E301 W305 A335 G375
• Catalytic site (residue number reindexed from 1): E289 W293 A323 G363
• Enzyme Commision number: 4.1.99.3: deoxyribodipyrimidine photo-lyase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	A160 H161 R164 N257 E301 W305 Y376 M379 W421 R429 W431 R441 Y442 M443 S444 R450 K451	A159 H160 R163 N245 E289 W293 Y364 M367 W409 R417 W419 R429 Y430 M431 S432 R438 K439	PDBbind-CN: Kd=44nM
BS02	dna	A	Y158 R429 A430 W431	Y157 R417 A418 W419	PDBbind-CN: Kd=44nM
BS03	FAD	A	Y252 S265 N266 S268 F298 E301 I302 W305 K306 K372 R378 N403 D409 N414 G415 I419	Y240 S253 N254 S256 F286 E289 I290 W293 K294 K360 R366 N391 D397 N402 G403 I407

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003677 DNA binding
• GO:0003904 deoxyribodipyrimidine photo-lyase activity
• GO:0016829 lyase activity

Biological Process

• GO:0000719 photoreactive repair
• GO:0006281 DNA repair

External links

• PDB: RCSB:2xrz, PDBe:2xrz, PDBj:2xrz
• PDBsum: 2xrz
• PubMed: 21892138
• UniProt: Q8PYK9